Chromatographic and biological properties of immunoreactive somatostatin in hypothalamic and extrahypothalamic brain regions of the rat.
Chromatographic and biological properties of immunoreactive somatostatin in hypothalamic and extrahypothalamic brain regions of the rat.
复制标题
大鼠下丘脑和下丘脑外脑区免疫反应性生长抑素的色谱和生物学特性。
DOI：
10.1210/endo-105-5-1083
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发表时间：
1979
期刊：
影响因子：
4.8
通讯作者：
Joseph B. Martin
中科院分区：
文献类型：
--
作者：
O. Rorstad;Jacques Epelbaum;Paul Brazeau;Joseph B. Martin
采用羊生长抑素抗血清，建立了免疫反应层析和免疫亲和层析方法，研究了羊脑组织中生长抑素的免疫反应性。从大鼠正中隆起、下丘脑前部-视前区、杏仁核和顶叶皮层提取的IRS可与免疫亲和柱可逆结合，提供了一种浓缩和部分纯化技术。免疫亲和纯化了四个脑区的IRS，在凝胶过滤层析上洗脱为四个峰。每个峰都具有生物活性，通过抑制培养的大鼠垂体前叶细胞释放生长激素来确定。下丘脑前部-视前区富含含有IRS的神经元细胞体，而中央隆起区IRS主要位于神经末梢，采用的方法未检测到IRS之间的差异。
A sheep antiserum to somatostatin was used to develop RIA and immunoaffinity chromatography methods for the study of immunoreactive somatostatin (IRS) in brain tissue. IRS extracted from rat median eminence, anterior hypothalamic-preoptic area, amygdala, and parietal cortex bound reversibly to immunoaffinity columns, providing a technique for concentration and partial purification. Immunoaffinity purified IRS from each of the four brain regions eluted as four peaks on gel filtration chromatography. Each peak possessed biological activity, as determined by inhibitory effects on the release of GH from cultured rat anterior pituitary cells. No differences were detected by the methods employed between IRS from the anterior hypothalamic-preoptic area, which is rich in IRS-containing neuronal cell bodies, and that from the median eminence, where IRS is localized predominantly in nerve terminals.