PURIFICATION OF HUMAN-MONOCYTES ON GELATIN-COATED SURFACES
PURIFICATION OF HUMAN-MONOCYTES ON GELATIN-COATED SURFACES
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DOI：
10.1016/0022-1759(86)90415-1
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发表时间：
1986-01-01
影响因子：
2.2
通讯作者：
DOUGLAS, SD
中科院分区：
文献类型：
--
作者：
HASSAN, NF;CAMPBELL, DE;DOUGLAS, SD
人外周血单核细胞分泌一种细胞膜相关糖蛋白--冷不溶性球蛋白(FN)。由于纤维连接蛋白结合到明胶涂层表面，我们开发了一种从整个单核细胞制备中分离人外周血单核细胞的简单技术。这些制剂的特点是单核细胞纯度高(90%以上)、低血小板污染和良好的存活率。
Human peripheral blood monocytes secrete a cell membrane-associated glycoprotein, cold insoluble globulin (fibronectin). Since fibronectin binds to gelatin-coated surface, we developed a simple technique for separation of human peripheral blood monocytes from whole mononuclear cell preparation. These preparations are characterized by a high monocyte purity (more than 90%), low platelet contamination and excellent viability.