SAFETY, IMMUNOGENICITY, AND EFFICACY OF A RECOMBINANTLY PRODUCED PLASMODIUM-FALCIPARUM CIRCUMSPOROZOITE-PROTEIN HEPATITIS-B SURFACE-ANTIGEN SUBUNIT VACCINE
SAFETY, IMMUNOGENICITY, AND EFFICACY OF A RECOMBINANTLY PRODUCED PLASMODIUM-FALCIPARUM CIRCUMSPOROZOITE-PROTEIN HEPATITIS-B SURFACE-ANTIGEN SUBUNIT VACCINE
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DOI：
10.1093/infdis/171.6.1576
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发表时间：
1995-06-01
影响因子：
6.4
通讯作者：
BALLOU, WR
中科院分区：
文献类型：
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作者：
GORDON, DM;MCGOVERN, TW;BALLOU, WR
20名未患疟疾的志愿者接受了含有19个NANP重复序列和在酵母中与肝炎B表面抗原共表达的环子孢子（CS）抗原的羧基末端（氨基酸210-398）的重组恶性疟原虫疟疾疫苗（RTS，S），10名接受了用明矾佐剂的疫苗，10名接受了用明矾加3-脱酰单磷酰脂质A（MPL）佐剂的疫苗。两种制剂均具有良好的耐受性和免疫原性。MPL增强CS抗体水平（通过ELISA、免疫荧光和子孢子侵入抑制测定法测量）。在子孢子攻击后，明矾组6/6和明矾-MPL组8/6发生了未闭疟疾。受保护的受试者在攻毒当天的C'S抗体滴度水平高于未受保护的受试者。免疫后，1名受保护的受试者对CS的细胞毒性T淋巴细胞活性增加，并回忆起对RTS、S和选择的CS的记忆T细胞应答。
Twenty malaria-naive volunteers received a recombinant Plasmodium falciparum malaria vaccine (RTS,S) containing 19 NANP repeats and the carboxy terminus (amino acids 210-398) of the circumsporozoite (CS) antigen coexpressed in yeast with hepatitis B surface antigen, Ten received vaccine adjuvanted with alum, and 10 received vaccine adjuvanted with alum plus 3-deacylated monophosphoryl lipid A (MPL). Both formulations were well tolerated and immunogenic. MPL enhanced CS antibody levels (measured by ELISA, immunofluorescence, and inhibition of sporozoite invasion assays). After sporozoite challenge, 6 of 6 in the alum group and 6 of 8 in the alum-MPL group developed patent malaria. Protected subjects had higher levels of C'S antibody titers on day of challenge than did nonprotected subjects. After immunization, 1 protected subject had increased cytotoxic T lymphocyte activity against CS and recall of memory T cell responses to RTS,S and selected CS.