High-fat diet alters gut microbiota physiology in mice
High-fat diet alters gut microbiota physiology in mice
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DOI：
10.1038/ismej.2013.155
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发表时间：
2014-02-01
期刊：
影响因子：
11
通讯作者：
Clavel, Thomas
中科院分区：
文献类型：
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作者：
Daniel, Hannelore;Gholami, Amin Moghaddas;Clavel, Thomas
已知肠道微生物群调节宿主能量稳态，并且可以受到高热量饮食的影响。然而，在功能层面影响生态系统的变化仍然没有得到很好的描述。我们测量了喂食碳水化合物或高脂肪（HF）饮食12周的小鼠盲肠细菌群落的变化，其水平如下：（i）通过高通量16S核糖体RNA基因测序的多样性和分类群分布;（ii）通过傅里叶变换红外-（FT-IR）和拉曼显微光谱法的本体和单细胞化学组成，以及（iii）通过高分辨率质谱法的元蛋白质组和代谢组。高脂饮食引起肠道优势细菌多样性的变化，并改变了瘤胃球菌科（减少）和Rikenellaceae（增加）的比例。FT-IR光谱显示，饮食对盲肠化学指纹的影响大于微生物群组成的影响。在单细胞水平上也观察到类杆菌目和毛螺菌科成员的生化指纹的饮食驱动变化，表明在不同饮食下优势菌类型的细胞组成存在明显差异。基于1760种细菌蛋白质和86种注释代谢物的元蛋白质组和代谢物组分析揭示了不同的HF饮食特异性谱。观察到激素和抗微生物网络、胆汁酸和胆红素代谢的改变以及向氨基酸和单糖代谢的转变。我们得出结论，HF饮食在功能水平上显著影响肠道细菌生态系统。
The intestinal microbiota is known to regulate host energy homeostasis and can be influenced by high-calorie diets. However, changes affecting the ecosystem at the functional level are still not well characterized. We measured shifts in cecal bacterial communities in mice fed a carbohydrate or high-fat (HF) diet for 12 weeks at the level of the following: (i) diversity and taxa distribution by high-throughput 16S ribosomal RNA gene sequencing; (ii) bulk and single-cell chemical composition by Fourier-transform infrared- (FT-IR) and Raman micro-spectroscopy and (iii) metaproteome and metabolome via high-resolution mass spectrometry. High-fat diet caused shifts in the diversity of dominant gut bacteria and altered the proportion of Ruminococcaceae (decrease) and Rikenellaceae (increase). FT-IR spectroscopy revealed that the impact of the diet on cecal chemical fingerprints is greater than the impact of microbiota composition. Diet-driven changes in biochemical fingerprints of members of the Bacteroidales and Lachnospiraceae were also observed at the level of single cells, indicating that there were distinct differences in cellular composition of dominant phylotypes under different diets. Metaproteome and metabolome analyses based on the occurrence of 1760 bacterial proteins and 86 annotated metabolites revealed distinct HF diet-specific profiles. Alteration of hormonal and anti-microbial networks, bile acid and bilirubin metabolism and shifts towards amino acid and simple sugars metabolism were observed. We conclude that a HF diet markedly affects the gut bacterial ecosystem at the functional level.