Modulation of vertebral and tibial growth by compression loading: diurnal versus full-time loading.
Modulation of vertebral and tibial growth by compression loading: diurnal versus full-time loading.
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通过压缩负荷调节椎骨和胫骨生长：昼夜负荷与全时负荷。
DOI：
10.1016/j.orthres.2004.06.012
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Aronsson,DavidD
中科院分区：
文献类型：
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作者：
Stokes,IanA;Gwadera,Jodie;Dimock,Abigail;Farnum,CorneliaE;Aronsson,DavidD
目的：本研究的目的是确定是否有量的endochondrium增长响应机械压缩和潜在的增长机制与夜间或白天的负载不同，相对于full-time loading.Methods：机械压缩（标称0.1 MPa应力）施加在胫骨和尾椎骨生长板的生长Sprague-Dawley大鼠。使用了4组动物（每组5只）：24/24 h（全时负荷）; 12/24 h（日间负荷）; 12/24 h（夜间负荷）;和0/24 h（假器械）。外侧胫骨和相邻椎骨作为动物内对照。8天后对动物实施安乐死。对生长板进行定量组织学处理，以测量24 h生长、总增殖区软骨细胞计数和BrdU阳性增殖区软骨细胞计数，以及生长方向上的肥大软骨细胞增大。结果：椎骨的生长百分比平均为动物内对照组的82%（全时）; 93%（日负荷）; 90%（夜间负荷）; 100%（假手术）。对于近端胫骨，平均为70%（全时）; 84%（日负荷）; 86%（夜间负荷）; 89%（假手术）。肥大性软骨细胞增大量的减少解释了全时加载生长板中约一半的这种效应，但在半时加载生长板中没有显著改变。生长的其余变化显然是由增殖区软骨细胞总数减少解释的。这些发现表明，在两个解剖位置，持续压缩负荷比间歇负荷抑制生长更多。© 2004骨科研究学会。由爱思唯尔有限公司出版。保留所有权利。
Purpose: This study was designed to determine whether the amount of endochondral growth response to mechanical compression and the underlying growth mechanism differed with night‐time or day‐time loading, relative to full‐time loading.Methods: Mechanical compression (nominally 0.1 MPa stress) was applied across tibial and tail vertebral growth plates of growing Sprague–Dawley rats. Four groups of animals (five per group) were used: 24/24 h (full‐time loading); 12/24 h (day‐loading); 12/24 h (night‐loading); and 0/24 h (sham instrumented). Contralateral tibiae and adjacent vertebrae served as within‐animal controls. The animals were euthanized after eight days. Growth plates were processed for quantitative histology to measure 24‐h growth, total and BrdU‐positive proliferative zone chondrocyte counts, and hypertrophic chondrocytic enlargement in the growth direction.Results: Growth as a percentage of within‐animal control averaged 82% (full‐time); 93% (day‐loading); 90% (night‐loading); 100% (sham) for vertebrae. For proximal tibiae it averaged 70% (full‐time); 84% (day‐loading); 86% (night‐loading); 89% (sham). Reduced amount of hypertrophic chondrocytic enlargement explained about half of this effect in full‐time loaded growth plates, but was not significantly altered in half‐time loaded growth plates. The remaining variation in growth was apparently explained by reduced total numbers of proliferative zone chondrocytes. These findings indicate that sustained compression loading suppressed growth more than intermittent loading at both anatomical locations. © 2004 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.