Genetic variation in VP7 gene of human rotavirus serotype 1 (G1 type) isolated in Japan and China.
Genetic variation in VP7 gene of human rotavirus serotype 1 (G1 type) isolated in Japan and China.
复制标题
日本和中国分离的人轮状病毒血清型 1（G1 型）VP7 基因的遗传变异。
DOI：
10.1006/viro.1993.1663
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发表时间：
1993
期刊：
影响因子：
3.7
通讯作者：
H. Ushijima
中科院分区：
文献类型：
--
作者：
K. Xin;S. Morikawa;Z. Fang;A. Mukoyama;K. Okuda;H. Ushijima
对日本和中国的12株轮状病毒血清1型分离株进行了编码主要中和糖蛋白（VP 7）的基因的序列分析。对血清1型分离株进行遗传变异检查。核苷酸和推导的氨基酸序列的比较研究表明，12个分离物和Wa株之间的总体同源性分别超过92%和96%。Wa与1979-1980年东京分离的2株（K1和K2）之间的同源性高于Wa与最近在东京和中国分离的菌株之间的同源性。在我们的分离株中，共有16个氨基酸残基频繁转化为另一种氨基酸。属于主要中和表位区的6个氨基酸残基（本通讯中的B、D和E）经常发生转化。根据这些数据，建议将其分为三个亚型（亚型A、B和中间型）。这些差异是否是轮状病毒流行病学中的重要机制还需要进一步研究。
Sequence analysis of the gene encoding the major neutralization glycoprotein (VP7) was performed on 12 human isolates of serotype 1 of rotavirus in Japan and China. They were examined for genetic variations among serotype 1 isolates. Comparative studies of their nucleotide and deduced amino acid sequences between the 12 isolates and the Wa strain revealed an overall homology of more than 92 and 96%, respectively. Higher degrees of homologies were observed between Wa and 2 strains (K1 and K2) in Tokyo, 1979-1980, than between Wa and recent isolated strains in Tokyo and in China. In our isolates, a total of 16 amino acid residues frequently converted to another amino acid. Six amino acid residues belonging to the major neutralizing epitope regions (B, D, and E in this communication) frequently converted. From these data three subtypes (subtypes A, B, and intermediate) were suggested to be divided. Whether these differences are an important mechanism in the epidemiology of rotaviruses requires further investigation.