Expression of Reactive Oxygen Species during Wound Healing of Vocal Folds in a Rat Model
Expression of Reactive Oxygen Species during Wound Healing of Vocal Folds in a Rat Model
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DOI：
10.1177/000348941212101206
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发表时间：
2012-12
期刊：
影响因子：
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通讯作者：
Masanobu Mizuta;S. Hirano;S. Ohno;I. Tateya;S. Kanemaru;Tatsuo Nakamura;J. Ito
中科院分区：
文献类型：
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作者：
Masanobu Mizuta;S. Hirano;S. Ohno;I. Tateya;S. Kanemaru;Tatsuo Nakamura;J. Ito
目的：以前的研究表明，虽然正常的伤口愈合需要低水平的活性氧（ROS），但过量的ROS会损害伤口愈合。在受伤的声带，这种过度可能会导致发音困难，由于疤痕，这是很难治疗的。然而，在声带伤口愈合过程中的ROS的表达还有待研究。在这项研究中，我们评估了ROS的表达和定位在受损的声带免疫组化分析。方法：经口内镜下剥除声带黏膜，造成SD大鼠单侧声带损伤。在损伤后的特定时间点收获喉，并对4-羟基-2-壬烯醛（4-HNE）（ROS标记物）和炎性细胞的存在进行化学检测。结果如下：我们发现，4-HNE-免疫阳性细胞显着增加，在固有层的损伤声带相比，正常声带在伤后第1和第3天。超过一半的4-HNE免疫阳性细胞也是巨噬细胞和粒细胞特异性抗体免疫阳性。结论：这项研究表明，大量的活性氧产生于早期阶段的伤口愈合，直到伤后第3天，这一时期可能是至关重要的调节活性氧水平。结果还表明，炎症细胞可能有助于ROS的产生。
Objectives: Previous studies have indicated that although normal wound healing requires low levels of reactive oxygen species (ROS), excessive amounts of ROS impair wound healing. In injured vocal folds, this excess may result in dysphonia due to scarring that is difficult to treat. However, the expression of ROS during vocal fold wound healing has yet to be investigated. In this study, we assessed the expression and localization of ROS in injured vocal folds by immunohistochemical analysis. Methods: Vocal folds of Sprague-Dawley rats were unilaterally injured by stripping the mucosa under transoral endoscopy. The larynges were harvested at specific time points after injury and were immunohistochemically examined for 4-hydroxy-2-nonenal (4-HNE), an ROS marker, and for the presence of inflammatory cells. Results: We found that 4-HNE–immunopositive cells were significantly increased in the lamina propria of the injured vocal folds as compared to the normal vocal folds on postinjury days 1 and 3. More than half of the 4-HNE–immunopositive cells were also immunopositive for a macrophage- and granulocyte-specific antibody. Conclusions: This study suggests that a large amount of ROS is produced during early-phase wound healing, until postinjury day 3, and that this period may be crucial for regulating ROS levels. The results also suggest that inflammatory cells may contribute to ROS generation.