The implications of proliferation and apoptosis for lung cancer metastasis.
The implications of proliferation and apoptosis for lung cancer metastasis.
复制标题
增殖和凋亡对肺癌转移的影响。
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
R. Koomagi
中科院分区：
文献类型：
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作者：
M. Volm;R. Koomagi
转移性扩散的发生取决于许多因素，包括患者的病情和肿瘤的性质。在这项研究中，分析了非小细胞肺癌患者的增殖和凋亡与淋巴结受累发生率之间的关系（n=215例）。为分析淋巴结转移与肿瘤增殖活性的关系，采用细胞周期分布（流式细胞术）、PCNA和细胞周期蛋白A的表达（免疫组化）进行检测。免疫组化法检测Fas、Fas配体、caspase-3、Bcl-2。在这个回顾性分析中，没有发现肿瘤的增殖活性和淋巴结状态之间的联系。相反，凋亡因子与淋巴结转移存在相关性。促凋亡因子Fas、Fas-配体和caspase-3的高表达与较低的淋巴结受累率相关（Fas-配体，p=0.004; caspase-3, p=0.007）。抗凋亡因子Bcl-2与肿瘤转移呈负相关的趋势与目前对Bcl-2基因功能的认识相吻合。所有患者的结果均可在鳞状细胞肺癌患者中得到证实。
The occurrence of metastatic spread depends on many factors both the condition of the patient and the properties of the tumor. In this investigation the association between proliferation and apoptosis and the incidence of lymph node involvement of patients with non-small cell lung carcinomas was analysed (n=215 patients). In order to analyse the relationship between lymph node metastasis and proliferative activity of the carcinomas, the distribution of cell cycle phases (flow cytometry), the expression of PCNA and cyclin A (immunohistochemistry) was determined. Fas, Fas-ligand, caspase-3 and Bcl-2 were determined by immunohistochemistry. In this retrospective analysis no association between proliferative activity of the tumors and lymph node status was found. In contrast, there existed a correlation between the apoptotic factors and lymph node metastasis. Higher expression of the pro-apoptic factors Fas, Fas-ligand and caspase-3 correlated with a lower incidence of lymph node involvement (Fas-ligand, p=0.004; caspase-3, p=0.007). The trend of an inverse correlation between the anti-apoptotic factor Bcl-2 and metastasis fits well into the present knowledge about the function of the bcl-2 gene. The results obtained from all the patients could be confirmed in patients with squamous cell lung carcinomas.