Lysophosphatidic acid activates phosphoinositide 3-kinase and phospholipase C in human platelets: inhibitory effects of Wortmannin on phosphoinositide 3-kinase and aggregation.
Lysophosphatidic acid activates phosphoinositide 3-kinase and phospholipase C in human platelets: inhibitory effects of Wortmannin on phosphoinositide 3-kinase and aggregation.
复制标题
溶血磷脂酸激活人血小板中的磷酸肌醇 3-激酶和磷脂酶 C：渥曼青霉素对磷酸肌醇 3-激酶和聚集的抑制作用。
DOI：
10.1006/bbrc.1995.1839
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Rittenhouse,SE
中科院分区：
文献类型：
--
作者：
Zhang,J;Rittenhouse,SE
溶血磷脂酸是一种具有生物活性的血清磷脂，已知具有生长因子样活性并引起血小板聚集。活化的磷酸肌醇3-激酶被认为参与细胞骨架重组和有丝分裂。我们报道溶血磷脂酸可激活血小板磷脂酰肌醇3-激酶，导致磷脂酰肌醇（3.4，5）P3和磷脂酰肌醇（3，4）P2的积累，并刺激磷脂酶C。Wortmannin是一种有效的磷酸肌醇3-激酶抑制剂，可阻断溶血磷脂酸诱导的血小板聚集，而不损害磷脂酶C的活化。作为一种纤维蛋白原与血小板整合素结合的拮抗剂，它能完全阻断血小板聚集，而不抑制磷酸肌醇3-激酶或磷脂酶C。我们认为，溶血磷脂酸，在激活磷脂酰肌醇3-激酶，促进血小板聚集，但血小板聚集反应溶血磷脂酸不显着增强磷脂酰肌醇3-激酶的激活。
Lysophosphatidic acid is a biologically active serum phospholipid known to have growth factor-like activities and to cause platelet aggregation. Activated phosphoinositide 3-kinase has been suggested to be involved in cytoskeletal reorganization and mitogenesis. We report that lysophosphatidic acid causes platelet phosphoinositide 3-kinase activation, leading to accumulation of phosphatidylinositol (3.4,5)P3and phosphatidylinositol (3,4)P2and stimulates phospholipase C. Wortmannin, a potent inhibitor of phosphoinositide 3-kinase, blocks platelet aggregation induced by lysophosphatidic acid without impairing phospholipase C activation. Eristostatin, an antagonist of fibrinogen binding to platelet integrin, completely blocks platelet aggregation without inhibiting phosphoinositide 3-kinase or phospholipase C. We suggest that lysophosphatidic acid, in activating phosphoinositide 3-kinase, promotes platelet aggregation, but that platelet aggregation in response to lysophosphatidic acid does not significantly enhance phosphoinositide 3-kinase activation.