Highly-sensitive and selective colorimetric sensor for amino acids chiral recognition based on molecularly imprinted photonic polymers
Highly-sensitive and selective colorimetric sensor for amino acids chiral recognition based on molecularly imprinted photonic polymers
复制标题
DOI：
10.1016/j.snb.2013.02.079
复制
发表时间：
2013-05
影响因子：
8.4
通讯作者：
Yin-Xia Zhang;Pinwen Zhao;Li‐Ping Yu
中科院分区：
文献类型：
--
作者：
Yin-Xia Zhang;Pinwen Zhao;Li‐Ping Yu
分子印迹材料与光子晶体的结合提供了一个理想的传感平台，在此平台上可以同时实现分析物识别和依赖分析物的转导方法。本文研制了一种比色分子印迹光子聚合物（MIPP）传感器，用于氨基酸的灵敏和选择性手性识别。该传感器是通过将前驱体和印迹分子渗透到聚苯乙烯光子晶体模板中，然后进行热聚合制备的。去除模板后，得到的MIPP由一个三维的、高度有序的、相互连接的大孔阵列组成。因此，氨基酸识别事件可以通过MIPP衍射峰位移直接转化为可见的颜色变化和可读的光信号。在pH为4时，l-焦谷氨酸（l-Pga）浓度与衍射峰位移在0.01 ~ 0.50mM范围内呈函数关系，当l-Pga浓度低于0.20mM时，衍射峰位移呈斜率为278nm/mM的线性关系。手性识别过程伴随着MIPP颜色的逐渐变化，肉眼可以很容易地观察到。该方法已应用于味精样品中l-Pga的检测。
The incorporation of molecularly imprinted materials into photonic crystals presents an ideal sensing platform on which analyte recognition and analyte-dependent transduction methodology could be realized simultaneously. Here, a colorimetric molecularly imprinted photonic polymer (MIPP) sensor has been developed for the sensitive and selective chiral recognition of amino acids. The sensor was fabricated by infiltrating a polystyrene photonic crystal template with precursor and imprinting molecules, followed by a thermal polymerization. After removal of templates, the resulted MIPP consists of a three-dimensional, highly-ordered and interconnected macroporous array. The amino acids recognition events thus could be directly transferred into visible color changes and readable optical signals through the diffraction peak shifts of MIPP. A function relationship was found between the diffraction peak shift and the concentration of l-pyroglutamic acid (l-Pga) in the range of 0.01–0.50mM at pH 4, and a linear relationship with a slope of 278nm/mM was found when the concentration of l-Pga was lower than 0.20mM. The chiral recognition process accompanying with a gradual color change of MIPP can be easily visualized by the naked eye. The developed method has been applied to detect l-Pga in monosodium glutamate samples.