Neural stem cells and neurogenesis in the adult zebrafish brain: Origin, proliferation dynamics, migration and cell fate
Neural stem cells and neurogenesis in the adult zebrafish brain: Origin, proliferation dynamics, migration and cell fate
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DOI：
10.1016/j.ydbio.2006.03.040
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发表时间：
2006-07-01
影响因子：
2.7
通讯作者：
Brand, Michael
中科院分区：
文献类型：
--
作者：
Grandel, Heiner;Kaslin, Jan;Brand, Michael
脊椎动物的终身神经发生依赖于干细胞产生增殖区，其中含有具有不同命运的神经元前体。成年斑马鱼脑中的增殖区位于沿其整个前后轴沿着的不同区域。我们显示了一个以前不受重视的程度的保护脑增殖模式之间的硬骨鱼，硬骨鱼地面计划的暗示。脉冲追踪标记的增殖人口揭示了离心运动的细胞远离他们的出生地到周围的地幔区。我们观察到切线迁移出生在腹侧端脑的细胞，但只有一个小的吻迁移流的嗅球。相反，端脑外侧区，一个被认为与哺乳动物齿状回同源的区域，显示出与哺乳动物一样的中间神经元的产生和迁移。经过46天的追踪，新生的高度移动的细胞已经移动到增殖区周围的核区域。它们通常显示HuC/D免疫反应性，但重要的是还显示更特异的神经元身份，如酪氨酸羟化酶、5-羟色胺和小清蛋白的免疫反应性所示。第二个增殖标记的应用使我们能够识别保留在增殖区中的标记保留、活跃循环的细胞。后者符合神经干细胞的两个关键标准：标记保留和自我更新。(c)2006年爱思唯尔公司All rights reserved.
Lifelong neurogenesis in vertebrates relies on stem cells producing proliferation zones that contain neuronal precursors with distinct fates. Proliferation zones in the adult zebrafish brain are located in distinct regions along its entire anterior-posterior axis. We show a previously unappreciated degree of conservation of brain proliferation patterns among teleosts, suggestive of a teleost ground plan. Pulse chase labeling of proliferating populations reveals a centrifugal movement of cells away from their places of birth into the surrounding mantle zone. We observe tangential migration of cells born in the ventral telencephalon, but only a minor rostral migratory stream to the olfactory bulb. In contrast, the lateral telencephalic area, a domain considered homologous to the mammalian dentate gyrus, shows production of interneurons and migration as in mammals. After a 46-day chase, newborn highly mobile cells have moved into nuclear areas surrounding the proliferation zones. They often show HuC/D immunoreactivity but importantly also more specific neuronal identities as indicated by immunoreactivity for tyrosine hydroxylase, serotonin and parvalbumin. Application of a second proliferation marker allows us to recognize label-retaining, actively cycling cells that remain in the proliferation zones. The latter population meets two key criteria of neural stem cells: label retention and self renewal. (c) 2006 Elsevier Inc. All rights reserved.