Synthesis and characterization of core-shell star copolymers for in vivo PET imaging applications
Synthesis and characterization of core-shell star copolymers for in vivo PET imaging applications
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DOI：
10.1021/bm7014152
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发表时间：
2008-04-01
影响因子：
6.2
通讯作者：
Hawker, Craig J.
中科院分区：
文献类型：
--
作者：
Fukukawa, Ken-ichi;Rossin, Raffaella;Hawker, Craig J.
通过活性自由基聚合合成核-壳型星型共聚物为三维纳米结构提供了一条便捷的途径，该结构具有聚乙二醇外壳、带有活性官能团的亲水性内壳和中心疏水性核心。通过从定义良好的线性二嵌段共聚物开始，可以精确控制每层的厚度、总体尺寸/分子量和内部反应官能团的数量，从而获得详细的结构/性能信息。这些聚合物纳米粒子与能够螯合放射性Cu-64核的dota配体的功能化使得这些材料的生物分布和体内正电子发射断层扫描（PET）成像得以研究，并与初始结构直接相关。结果表明，PEG壳层厚度增加的纳米颗粒血液循环增加，排泄器官蓄积少，提示其作为体内载体应用于成像、靶向和治疗组。
The synthesis of core-shell star copolymers via living free radical polymerization provides a convenient route to three-dimensional nanostructures having a poly(ethylene glycol) outer shell, a hydrophilic inner shell bearing reactive functional groups, and a central hydrophobic core. By starting with well-defined linear diblock copolymers, the thickness of each layer, overall size/molecular weight, and the number of internal reactive functional groups can be controlled accurately, permitting detailed structure/performance information to be obtained. Functionalization of these polymeric nanoparticles with a DOTA-ligand capable of chelating radioactive Cu-64 nuclei enabled the biodistribution and in vivo positron emission tomography (PET) imaging of these materials to be studied and correlated directly to the initial structure. Results indicate that nanoparticles with increasing PEG shell thickness show increased blood circulation and low accumulation in excretory organs, suggesting application as in vivo carriers for imaging, targeting, and therapeutic groups.