Influences of endogenous and exogenous TGF-beta on elastin in rat lung fibroblasts and aortic smooth muscle cells.
Influences of endogenous and exogenous TGF-beta on elastin in rat lung fibroblasts and aortic smooth muscle cells.
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内源性和外源性TGF-β对大鼠肺成纤维细胞和主动脉平滑肌细胞弹性蛋白的影响。
DOI：
10.1152/ajplung.1992.263.2.l257
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
McGowan,SE
中科院分区：
文献类型：
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作者：
McGowan,SE
在新生儿肺发育过程中调节弹性蛋白产生的因素尚未阐明。以前的研究表明，转化生长因子-β（TGF-β）增加新生大鼠肺成纤维细胞（LF）的弹性蛋白产生。我们研究了TGF-β的这种作用是否是这些细胞所独有的，或者在其他新生儿细胞中是明显的，这些新生儿细胞组成性地产生弹性蛋白，或者在成人细胞中，其组成性弹性蛋白产生低。我们已经定量的可溶性弹性蛋白，弹性蛋白mRNA，和TGF-β的生产在原代培养的平滑肌细胞（SMC）和LF从新生儿和成年大鼠，并检查了可溶性弹性蛋白和弹性蛋白mRNA的变化，导致添加100 pM外源性TGF-β 1到这些文化。从新生大鼠获得的LF和SMC的无补充的文化表现出较高的稳态水平的弹性蛋白mRNA，并含有更多的可溶性弹性蛋白在其培养基中比成年动物的细胞。当新生儿LF补充100 pM TGF-β 1，他们表现出显着增加，其培养基中的可溶性弹性蛋白含量和稳态弹性蛋白mRNA。无论是从成人获得的LF，也没有从新生儿或成年大鼠获得的SMC显着增加其可溶性弹性蛋白或稳态弹性蛋白mRNA后，加入外源性TGF-β。当新生儿LF补充抗TGF-β中和抗体，可溶性弹性蛋白含量的培养基显着下降。这些数据表明，弹性蛋白表达对TGF-β的反应仅限于新生儿LF，内源性TGF-β影响新生儿LF的弹性蛋白产生。
The factors that regulate elastin production during neonatal lung development have not been elucidated. Previous investigations suggested that transforming growth factor-beta (TGF-beta) increases elastin production by neonatal rat lung fibroblasts (LF). We examined whether this effect of TGF-beta was unique to these cells or was evident in other neonatal cells, which constitutively produce elastin, or in cells from adults, whose constitutive elastin production is low. We have quantitated soluble elastin, elastin mRNA, and TGF-beta production in primary cultures of smooth muscle cells (SMC) and LF from neonatal and adult rats and have examined the alterations in soluble elastin and elastin mRNA that result from adding 100 pM exogenous TGF-beta 1 to these cultures. Unsupplemented cultures of LF and SMC obtained from neonatal rats exhibited higher steady-state levels of elastin mRNA and contained more soluble elastin in their culture medium than did cells from adult animals. When neonatal LF were supplemented with 100 pM TGF-beta 1, they showed a significant increase in the soluble elastin content of their culture medium and their steady-state elastin mRNA. Neither LF obtained from adults nor SMC obtained from neonatal or adult rats significantly increased their soluble elastin or steady-state elastin mRNA after the addition of exogenous TGF-beta. When neonatal LF were supplemented with an anti-TGF-beta neutralizing antibody, the soluble elastin content of the culture medium decreased significantly. These data suggest that the responsiveness of elastin expression to TGF-beta is limited to neonatal LF and that endogenous TGF-beta influences elastin production by neonatal LF.