Screening of BC1F1 population (BG 379-2/ IR 07F102 // BG 379-2) of rice (Oryza sativa L.) for submergence tolerance using molecular markers
Screening of BC1F1 population (BG 379-2/ IR 07F102 // BG 379-2) of rice (Oryza sativa L.) for submergence tolerance using molecular markers
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使用分子标记筛选水稻 (Oryza sativa L.) 的 BC1F1 群体 (BG 379-2/ IR 07F102 // BG 379-2) 的耐淹性
DOI：
10.4038/jas.v9i3.7437
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发表时间：
2014
影响因子：
0.6
通讯作者：
W. Samarasinghe
中科院分区：
文献类型：
--
作者：
Rn Nawarathna;A. Perera;W. Samarasinghe
水稻从发芽到早期营养阶段易受淹没，并影响湿地水稻种植区。当地没有耐淹品种。因此，本研究旨在评估BC 1F 1群体（Bg 379-2/ IR 07 F102// Bg 379-2）的耐淹性进行形态学鉴定，并筛选出供体亲本IR 07 F102与轮回亲本Bg 379-2之间的多态性分子标记，利用已鉴定的分子标记对BC 1 F1群体进行耐淹性筛选。利用5个水稻微卫星标记和1个InDel标记对108个水稻BC 1F 1单株进行了植株倒伏、叶片衰老和植株伸长的形态学筛选和分子标记筛选。其中，利用分子标记RM 464 A对具有耐淹涝性的SUB 1基因进行了筛选。通过形态学和分子生物学鉴定，筛选出5株耐淹单株。DOI：http://dx.doi.org/10.4038/jas.v9i3.7437农业科学杂志，2014年，第9卷，第3期：第147 -153页
Rice is susceptible to submergence from germination to early vegetative stage and affects wetland paddy cultivation areas of Sri Lanka is affected by flash floods. There are no submergence tolerant varieties available locally. Therefore, this research was designed to evaluate the BC 1 F 1 population (Bg 379-2/ IR 07F102 // Bg 379-2) morphologically for submergence tolerance and to select polymorphic molecular markers between the donor parents IR07F102 and recurrent parent, Bg 379-2, and to screen and select plants of the BC 1 F 1 population for submergence tolerance using identified molecular markers. The 108 individuals of BC 1 F 1 of rice were morphologically screened for plant lodging, leaf senescence and plant elongation and molecular screening was done by using 5 Rice microsatellites and 1 In Del marker. Out of those, molecular marker, RM464A for SUB1 gene conferring submergence tolerance, was used to screen the BC 1 F 1 population. Five plants were selected as submergence tolerant individuals as they were positive in both morphological and molecular screening. DOI: http://dx.doi.org/10.4038/jas.v9i3.7437 The Journal of Agricultural Sciences, 2014, vol.9, no3: p.147-153