AN EX-VIVO RAT RETINAL PREPARATION FOR EXCITOTOXICITY STUDIES
AN EX-VIVO RAT RETINAL PREPARATION FOR EXCITOTOXICITY STUDIES
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DOI：
10.1016/0165-0270(95)00015-m
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发表时间：
1995-08-01
影响因子：
3
通讯作者：
OLNEY, JW
中科院分区：
文献类型：
--
作者：
IZUMI, Y;BENZ, AM;OLNEY, JW
虽然分离的鸡胚视网膜是研究兴奋性毒性机制的非常有用的体外制剂，但它是鸟类而不是哺乳动物组织，其胚胎年龄使其不适合进行全面的发育和衰老研究。因此，我们探索了利用不同年龄的大鼠视网膜进行体外兴奋毒性研究的可行性。在该模型中，视网膜节段在5摄氏度的人工脑脊液中分离，然后在30摄氏度的不同条件下孵育，并进行组织学评估神经退行性变化的迹象。在脑脊液中孵育3h的7、30、120和660日龄大鼠的视网膜切片和孵育24 h的30日龄大鼠的视网膜切片保持正常的组织学外观。因此，该制剂适合于从婴儿期到老年的任何年龄的哺乳动物中枢神经系统的急性或迟发性兴奋毒性现象的体外研究。在30日龄大鼠视网膜上进行的激动剂实验显示，令人惊讶的结果是，非NMDA激动剂Kainate和AMPA在低浓度(100 MM)时对视网膜神经元的损伤远远多于NMDA在很高浓度(10 MM)时对视网膜神经元的损伤。
Although the isolated chicken embryo retina has been a very useful in vitro preparation for studying mechanisms of excitotoxicity, it is an avian rather than mammalian tissue and its embryonic age makes it unsuitable for a full range of developmental and aging studies. Therefore, we have explored the feasibility of using the rat retina at various ages for in vitro excitotoxicity studies. In this model, retinal segments were isolated in artificial cerebrospinal fluid (CSF) at 5 degrees C then incubated under various conditions at 30 degrees C and assessed histologically for signs of neurodegenerative changes. Retinal segments from 7-, 30-, 120- and 660-day-old rats incubated in CSF for 3 h and from 30-day-old rats incubated for 24 h retained a normal histological appearance. Thus, this preparation is suitable for in vitro studies pertaining to either acute or delayed excitotoxic phenomena in the mammalian CNS at any age from infancy to old age. Excitotoxin agonist experiments in the 30-day-old rat retina revealed the surprising result that the non-NMDA agonists, kainate and AMPA, at a low concentration (100 mu M) damaged a much larger number of retinal neurons than NMDA did at a very high concentration (10 mM).