Dietary fatty acids differentially modulate messenger RNA abundance of low-density lipoprotein receptor, 3-hydroxy-3-methylglutaryl coenzyme A reductase, and microsomal triglyceride transfer protein in Golden-Syrian hamsters
Dietary fatty acids differentially modulate messenger RNA abundance of low-density lipoprotein receptor, 3-hydroxy-3-methylglutaryl coenzyme A reductase, and microsomal triglyceride transfer protein in Golden-Syrian hamsters
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DOI：
10.1016/j.metabol.2005.12.005
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发表时间：
2006-05-01
影响因子：
9.8
通讯作者：
Lichtenstein, AH
中科院分区：
文献类型：
--
作者：
Dorfman, SE;Lichtenstein, AH
膳食脂肪酸调节血浆和细胞内胆固醇浓度。循环非高密度脂蛋白胆固醇（nHDL-C）浓度由肝脏极低密度脂蛋白组装和分泌的速率以及随后代谢产物的清除率决定。膳食脂肪的影响在血浆脂质、肝脂质组成和低密度脂蛋白（LDL）受体、3-羟基3-甲基戊二酰辅酶A（HMG-CoA）还原酶、固醇调节元件结合蛋白（SREBP）2、和微粒体甘油三酯转移蛋白（MTP）（Charles River Laboratories，威尔明顿，MA）。用非纯化饮食（6.25脂肪g/100 g）与0.1 g胆固醇/100 g（对照饮食）或对照饮食与另外10 g实验脂肪/100 g喂养仓鼠12周。与黄油相比，喂食对照饮食、不饱和脂肪（菜籽油和大豆油）和人造黄油的仓鼠总胆固醇、nHDL-C和甘油三酯浓度显著降低。额外的膳食脂肪，无论脂肪酸谱，导致更高的肝脏胆固醇浓度。相比之下，相对于对照饮食，黄油，或人造黄油喂养的仓鼠，这些变化与高4倍和8倍的LDL受体和高5倍和9倍的SREBP mRNA丰度，分别与菜籽油和大豆油喂养的仓鼠。MTP mRNA是极低密度脂蛋白颗粒形成的标志物，相对于对照饮食喂养的仓鼠，在菜籽油和大豆油喂养的仓鼠中较高，尽管差异不大。这些结果表明，菜籽油和大豆油替代黄油导致nHDL-C浓度降低，这可能与LDL受体、SREBP-2和MTP基因的mRNA丰度增加有关。(c)2006年爱思唯尔公司All rights reserved.
Dietary fatty acids modulate plasma and intracellular cholesterol concentrations. Circulating non-high-density lipoprotein cholesterol (nHDL-C) concentration is determined by rates of hepatic very low-density lipoprotein assembly and secretion, and clearance of subsequent metabolic products. The effect of dietary fat (butter, traditional margarine, soybean oil, and canola oil) was assessed with respect to plasma lipids, hepatic lipid composition, and messenger RNA (mRNA) abundance of low-density lipoprotein (LDL) receptor, 3-hydroxy3-methylglutaryl coenzyme A (HMG-CoA) reductase, sterol regulatory element-binding protein (SREBP) 2, and microsomal triglyceride transfer protein (MTP) in the Golden-Syrian hamster (Charles River Laboratories, Wilmington, MA). Hamsters were fed with a nonpurified diet (6.25 fat g/100 g) with 0.1 g cholesterol/100 g (control diet) or control diet with an additional 10 g experimental fat/100 g for 12 weeks. Hamsters fed with the control diet, unsaturated fats (canola and soybean oils), and margarine, relative to butter, had significantly lower total cholesterol and nHDL-C and triglyceride concentrations. Additional dietary fat, regardless of fatty acid profile, resulted in higher hepatic cholesterol concentrations. In contrast, relative to the control diet-, butter-, or margarine-fed hamsters, these changes were associated with a 4- and 8-fold higher LDL receptor and 5- and 9-fold higher SREBP mRNA abundance, in hamsters fed with canola and soybean oils, respectively. MTP mRNA, a marker of very low-density lipoprotein particle formation; was higher in canola- and soybean oil-fed hamsters relative to the control diet-fed hamsters, although differences were modest. These results suggest that the substitution of canola and soybean oils for butter results in lower nHDL-C concentrations that may be related to increased mRNA abundance of the LDL receptor, SREBP-2, and MTP genes. (c) 2006 Elsevier Inc. All rights reserved.