HIGH PREALBUMIN AND TRANSFERRIN MESSENGER-RNA LEVELS IN THE CHOROID-PLEXUS OF RAT-BRAIN
HIGH PREALBUMIN AND TRANSFERRIN MESSENGER-RNA LEVELS IN THE CHOROID-PLEXUS OF RAT-BRAIN
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DOI：
10.1016/s0006-291x(85)80027-9
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发表时间：
1985-01-01
影响因子：
3.1
通讯作者：
SCHREIBER, G
中科院分区：
文献类型：
--
作者：
DICKSON, PW;ALDRED, AR;SCHREIBER, G
通过将放射性[互补] cDNA 与细胞质提取物中的 RNA 杂交，研究了大鼠大脑各个部位的血浆蛋白基因的表达。在大脑中没有检测到 .beta 的 mRNA。纤维蛋白原亚基、主要急性期α1-蛋白、α1-酸性糖蛋白和白蛋白。然而，每克组织中，脉络丛含有的前白蛋白 mRNA 含量至少比肝脏多 100 倍，转铁蛋白 mRNA 含量与肝脏大致相同。在大脑的其他区域没有发现前白蛋白 mRNA。结果表明，脉络丛中前白蛋白的合成非常活跃，这可能是甲状腺激素通过脑脊液从血液输送到大脑的重要环节。
Expression of plasma protein genes in various parts of the rat brain was studied by hybridizing radioactive [complementary] cDNA to RNA in cytoplasmic extracts. No mRNA could be detected in brain for the .beta. subunit of fibrinogen, major acute phase .alpha.1-protein, .alpha.1-acid glycoprotein and albumin. However, per gram tissue, the choroid plexus contained at least 100 times larger amounts of prealbumin mRNA than the liver and about the same amount of transferrin mRNA as liver. No prealbumin mRNA was found in other areas of the brain. The results suggest very active synthesis of prealbumin in the choroid plexus, which may be an important link in the transport of thyroid hormones from the blood to the brain via the CSF.