Biosynthesis of D-glucaric acid from sucrose with routed carbon distribution in metabolically engineered Escherichia coli
Biosynthesis of D-glucaric acid from sucrose with routed carbon distribution in metabolically engineered Escherichia coli
复制标题
在代谢工程大肠杆菌中利用路由碳分布从蔗糖生物合成 D-葡萄糖酸
DOI：
10.1016/j.ymben.2018.04.020
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发表时间：
2018-05-01
影响因子：
8.4
通讯作者：
Fan, Li-Hai
中科院分区：
文献类型：
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作者：
Qu, Ya-Nan;Yan, Hao-Jie;Fan, Li-Hai
葡萄糖二酸是一种很有前途的平台化合物，可用于合成许多其他高附加值或商品化学品。将D-葡萄糖有效转化为D-葡糖二酸的大肠杆菌工程化已经尝试了几年，由于D-葡糖二酸产量的增加，混合糖发酵最近获得了越来越多的关注。在这里，我们在E. coll. BL 21（DE 3），在功能上构建了从蔗糖到D-葡糖二酸的未报道的途径。染色体zwf、pgi、ptsG、uxaC、gudD的进一步缺失、glk的过表达以及对pgi使用D-果糖依赖性翻译控制系统使得菌株能够使用蔗糖作为唯一碳源，同时实现高产物滴度和产率。在含10 g/L蔗糖的M9培养基中，D-葡萄糖二酸的效价可达1.42 g/L，产量可达0.142 g/g。
D-glucaric acid is a promising platform compound used to synthesize many other value-added or commodity chemicals. The engineering of Escherichia coll. for efficiently converting D-glucose to D-glucaric acid has been attempted for several years, with mixed sugar fermentation recently gaining growing interests due to the increased D-glucaric acid yield. Here, we co-expressed cscB, cscA, cscK, ino1, miox, udh, and suhB in E. coll. BL21 (DE3), functionally constructing an unreported route from sucrose to D-glucaric acid. Further deletion of chromosomal zwf, pgi, ptsG, uxaC, gudD, over-expression of glk, and use of a D-fructose-dependent translation control system for pgi enabled the strain to use sucrose as the sole carbon source while achieving a high product titer and yield. The titer of D-glucaric acid in M9 medium containing 10 g/L sucrose reached similar to 1.42 g/L, with a yield of similar to 0.142 g/g on sucrose.