rRNA adenine methylation requires T07A9.8 gene as rram-1 in Caenorhabditis elegans
rRNA adenine methylation requires T07A9.8 gene as rram-1 in Caenorhabditis elegans
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秀丽隐杆线虫中 rRNA 腺嘌呤甲基化需要 T07A9.8 基因作为 rram-1
DOI：
10.1093/jb/mvy018
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Fukamizu Akiyoshi
中科院分区：
文献类型：
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作者：
Yokoyama Wataru;Hirota Keiko;Wan Huahua;Sumi Naoaki;Miyata Mai;Araoi Sho;Nomura Naoto;Kako Koichiro;Fukamizu Akiyoshi
RNA在所有生命王国中都是转录后修饰的。在这些修饰中，碱基甲基化在真核生物核糖体RNA（rRNA）中高度保守。最近，rRNA加工蛋白8（Rrp 8）和核甲基素（NML）分别被鉴定为酵母25 S和哺乳动物28 S rRNA中N1-甲基腺苷（m1A）修饰的因子。然而，在秀丽隐杆线虫中，rRNA的m1A修饰仍然知之甚少（C. elegans）。在这里，我们使用液相色谱/串联质谱分析和RNA免疫沉淀分析，我们已经确定了m1A修饰位于26 S rRNA inC的674位（A674）附近。优雅的。此外，基于定量PCR的分析显示T07A9.8、aC.酵母Rrp 8和人NML的eleganshomolog负责26 S rRNA的A674处的m1A修饰。该m1A修饰位点位于C.与酵母25 S rRNA和人28 S rRNA的序列一致。有趣的是，T07A9.8在正常营养条件下与前rRNA转录无关。由于26 S rRNA的m1A修饰需要T07A9.8 inC。我们将该基因命名为rRNAadeninemethyltransferase-1（rram-1）。
RNAs are post-transcriptionally modified in all kingdoms of life. Of these modifications, base methylations are highly conserved in eukaryote ribosomal RNA (rRNA). Recently, rRNA processing protein 8 (Rrp8) and nucleomethylin (NML) were identified as factors ofN1-methyladenosine (m1A) modification in yeast 25 S and mammalian 28 S rRNA, respectively. However, m1A modification of rRNA is still poorly understood inCaenorhabditis elegans(C. elegans). Here, using the liquid chromatography/tandem mass spectrometry analysis and RNA immunoprecipitation assay, we have identified that the m1A modification is located around position 674 (A674) of 26 S rRNA inC. elegans. Furthermore, quantitative PCR-based analysis revealed that T07A9.8, aC. eleganshomolog of yeast Rrp8 and human NML, is responsible for m1A modification at A674 of 26 S rRNA. This m1A modification site inC. eleganscorresponds to those in yeast 25 S rRNA and human 28 S rRNA. Intriguingly, T07A9.8 is not associated with pre-rRNA transcription under normal nutrient conditions. Since the m1A modification of 26 S rRNA requires T07A9.8 inC. elegans, we designated the gene asrRNAadeninemethyltransferase-1(rram-1).