Performance of serial time-encoded amplified microscope
Performance of serial time-encoded amplified microscope
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DOI：
10.1364/oe.18.010016
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发表时间：
2010-05-10
期刊：
影响因子：
3.8
通讯作者：
Jalali, Bahram
中科院分区：
文献类型：
--
作者：
Tsia, Kevin K.;Goda, Keisuke;Jalali, Bahram
序列时间编码放大显微镜(STEAM)是一种全新的成像方式，可以实现高灵敏度的超快连续实时成像。通过光学图像放大，STEAM克服了影响几乎所有光学成像系统的灵敏度和速度之间的基本权衡。与传统的显微镜系统不同，STEAM的性能不仅取决于透镜，还取决于STEAM特有的其他组件的性质，即空间分散器、群速度色散元件和后端电子数字化装置。在这篇文章中，我们提出了一种分析，说明了这些因素是如何影响空间分辨率的，以及它们如何在像素数和蒸汽成像仪的帧速率之间产生权衡。我们还量化了STEAM的光学图像放大功能如何提高成像灵敏度。这些分析不仅为STEAM技术的运行提供了有价值的见解，也为这一新成像技术的实施和优化提供了蓝图。(C)2010年美国光学学会
Serial time-encoded amplified microscopy (STEAM) is an entirely new imaging modality that enables ultrafast continuous real-time imaging with high sensitivity. By means of optical image amplification, STEAM overcomes the fundamental tradeoff between sensitivity and speed that affects virtually all optical imaging systems. Unlike the conventional microscope systems, the performance of STEAM depends not only on the lenses, but also on the properties of other components that are unique to STEAM, namely the spatial disperser, the group velocity dispersion element, and the back-end electronic digitizer. In this paper, we present an analysis that shows how these considerations affect the spatial resolution, and how they create a trade-off between the number of pixels and the frame rate of the STEAM imager. We also quantify how STEAM's optical image amplification feature improves the imaging sensitivity. These analyses not only provide valuable insight into the operation of STEAM technology but also serve as a blue print for implementation and optimization of this new imaging technology. (C)2010 Optical Society of America