Reassignment of Enterobacter dissolvens to Enterobacter cloacae as E-cloacae subspecies dissolvens comb. nov and emended description of Enterobacter asburiae and Enterobacter kobei
Reassignment of Enterobacter dissolvens to Enterobacter cloacae as E-cloacae subspecies dissolvens comb. nov and emended description of Enterobacter asburiae and Enterobacter kobei
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DOI：
10.1016/j.syapm.2004.12.010
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发表时间：
2005-04-01
影响因子：
3.4
通讯作者：
Roggenkamp, A
中科院分区：
文献类型：
--
作者：
Hoffmann, H;Stindl, S;Roggenkamp, A
通过对模式菌株ATCC 23373 T和3株临床分离菌株的分析，重新评价了分离肠杆菌的分类地位。在最近的群体遗传学研究框架下，通过系统发育序列分析，将这些菌株归入该物种的遗传聚类群。通过DNA-DNA杂交研究，分析了E溶物与阴沟肠杆菌复合体其他物种的亲缘关系。E dissolvens的遗传聚类群与阴沟肠杆菌属于同一个基因相关群，平均ΔT-m值为3.9C，证实了前三项研究的杂交结果。对分离的阴沟肠杆菌和溶解阴沟肠杆菌的115个生化反应的表型分析表明，七叶皂苷试验是区分它们的唯一方法，对溶解肠杆菌呈阳性反应，对阴沟肠杆菌呈阴性反应。命名为阴沟肠杆菌亚种。溶解梳子。11月建议用于以前称为E dissolvens的生物群，并将其命名为E Cloacae亚种。泄殖腔梳子。11月对于以前称为阴沟肠杆菌的生物群。根据收集的17株和15株细菌的数据，分别修订了科贝肠杆菌和阿斯伯里肠杆菌的种类描述。通过系统发育序列分析和DNA-DNA杂交，将这些菌株归类为各自的物种。115个反应的表型分析给出了具有新的区分表型特性的详细的物种概况。©2004爱思唯尔股份有限公司。版权所有。
The taxonomic position of Enterobacter dissolvens was re-evaluated based on the analysis of the type strain ATCC 23373 T and three clinical isolates. The strains were assigned to the genetic cluster of the species by phylogenetic sequence analysis in the frame of a recent population genetic study. The relatedness of E dissolves to the other species of the E. cloacae complex was analyzed by DNA-DNA hybridization studies based on melting profiles in microplates. The genetic cluster of E dissolvens fell into the same DNA-relatedness group like E cloacae with mean Δ T-m-values of 3.9 ° C confirming the hybridization results of three former studies. Phenotypic analysis of the E cloacae and E dissolvens strains, respectively, based on 115 biochemical reactions yielded the esculin test as the only one differentiating between them by being positive for E dissolvens and negative for E cloacae strains. The name E cloacae subsp. dissolvens comb. nov. is proposed for the group of organisms formerly referred to as E dissolvens, and the name E cloacae subsp. cloacae comb. nov. for the group of organisms formerly referred to as E cloacae. The species descriptions of Enterobacter kobei and Enterobacter asburiae were emended based on the data collected on 17 and 15 strains, respectively. The strains were assigned to the respective species by a combination of phylogenetic sequence analyzes and DNA-DNA hybridizations. Phenotypic analyzes of 115 reactions gave detailed species profiles with new differentiating phenotypic properties. © 2004 Elsevier GmbH. All rights reserved.