The Effect of Zinc, Arginine, Fructose and Seminal Supernatant of Normal Semen on the Triple Adenosine Triphosphatase Activities of the Spermatozoa from Males with Oligoasthenozoospermia
The Effect of Zinc, Arginine, Fructose and Seminal Supernatant of Normal Semen on the Triple Adenosine Triphosphatase Activities of the Spermatozoa from Males with Oligoasthenozoospermia
复制标题
锌、精氨酸、果糖及正常精液精上清液对少弱精子症男性精子三磷酸腺苷酶活性的影响
作者：
M. Karacagil;M. Sade;R. Türkyilmaz
摘要： 当少弱精子症患者的精子与正常精液的上清液悬浮时，除了精子活力增强之外，还观察到三重 ATP 酶活性增加。这表明正常精液上清液中存在的影响精子活力的一个或多个因素也对三重 ATP 酶活性具有积极影响。为了产生这种效果，将锌、精氨酸和果糖添加到培养介质中，并在其中测定精子 ATP 酶活性。锌增加Ca2+-Mg2+ ATP酶活性而不影响Na+/K+-Mg2+和Mg2+ ATP酶活性。添加精氨酸和果糖后，三重 ATP 酶活性保持不变。因此，锌被认为是影响精浆中精子活力的因素之一。
Summary: When the spermatozoa of oligoasthenozoospermic patients were suspended with the supernatant of normal semen, an increase in triple ATPase enzyme activities besides enhancement of spermatozoa motilities were observed. This suggests that factor or factors present in the supernatant of normal semen that effects spermatozoa motility also have a positive effect on triple ATPase enzyme activities. In an attempt to produce such an effect, zinc, arginine and fructose were added to the incubation media where the spermatozoal ATPase enzyme activities were determined. Zinc increased Ca2+‐Mg2+ ATPase enzyme activity without affecting Na+/K+‐Mg2+ and Mg2+ ATPase activities. Triple ATPase enzyme activities remained unchanged after arginine and fructose additions. As a result zinc is thought to be one of the factors that affect spermatozoa motility in seminal plasma.