Molecular Aptamer Beacons and Their Applications in Sensing, Imaging, and Diagnostics
Molecular Aptamer Beacons and Their Applications in Sensing, Imaging, and Diagnostics
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DOI：
10.1002/smll.201902248
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发表时间：
2019-07-17
期刊：
影响因子：
13.3
通讯作者：
Deo, Sapna K.
中科院分区：
文献类型：
--
作者：
Moutsiopoulou, Angeliki;Broyles, David;Deo, Sapna K.
监测不同生物分子的类型、浓度和活性的能力对于获得有关细胞内分子过程的信息至关重要。成功的监测需要一个敏感和选择性的工具，可以响应这些分子变化。分子适体信标（MAB）是一种分子成像和检测工具，通过结合分子信标和适体技术的选择性和灵敏度，可以实现小分子或大分子的可视化。MAB设计利用结构切换和特定识别，在目标存在的情况下产生光学开/关开关。各种供体猝灭剂对，如荧光染料，量子点，碳基材料，和金属纳米粒子已被用于单克隆抗体的设计。在这项工作中，MAB技术的各种生物医学应用的重点。不同的共轭策略的能量供体-受体对，并讨论了每个检测系统的整体灵敏度。该技术在生物医学研究和诊断领域的未来潜力也得到了强调。
The ability to monitor types, concentrations, and activities of different biomolecules is essential to obtain information about the molecular processes within cells. Successful monitoring requires a sensitive and selective tool that can respond to these molecular changes. Molecular aptamer beacon (MAB) is a molecular imaging and detection tool that enables visualization of small or large molecules by combining the selectivity and sensitivity of molecular beacon and aptamer technologies. MAB design leverages structure switching and specific recognition to yield an optical on/off switch in the presence of the target. Various donor-quencher pairs such as fluorescent dyes, quantum dots, carbon-based materials, and metallic nanoparticles have been employed in the design of MABs. In this work, the diverse biomedical applications of MAB technology are focused on. Different conjugation strategies for the energy donor-acceptor pairs are addressed, and the overall sensitivities of each detection system are discussed. The future potential of this technology in the fields of biomedical research and diagnostics is also highlighted.