Normalization of two-dimensional electrophoretic patterns of human plasma proteins and comparisons of cerebrospinal fluid and urine patterns in terms of the normalized map.
Normalization of two-dimensional electrophoretic patterns of human plasma proteins and comparisons of cerebrospinal fluid and urine patterns in terms of the normalized map.
复制标题
人血浆蛋白二维电泳图谱的标准化以及根据标准化图谱比较脑脊液和尿液图谱。
DOI：
10.1093/oxfordjournals.jbchem.a133267
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发表时间：
1981
影响因子：
2.7
通讯作者：
T. Okuyama
中科院分区：
文献类型：
--
作者：
T. Manabe;K. Kojima;S. Jitzukawa;T. Hoshino;T. Okuyama
通过比较正常人血浆的8个二维电泳凝胶，制备了人血浆蛋白的“归一化图谱”，其说明了蛋白的标准分布模式。检测了几种血浆蛋白的表型。计算归一化图谱中每个点的pI和分子量，并通过几种方法（包括从染色凝胶片提取的蛋白质的微量氨基酸分析）鉴定或初步鉴定128个点中的84个点为已知血浆蛋白。将人脑脊液和尿蛋白模式与标准化图谱进行比较，并确定每个体液样品的特征蛋白的位置。
A "normalized map" of human plasma proteins, which illustrated the standard distribution pattern of the proteins, was prepared by comparing eight two-dimensional electrophoretic gels of normal human plasma. Phenotypes of several plasma proteins were detected. The pI and molecular weight of each spot in the normalized map were calculated and 84 spots out of 128 were identified or tentatively identified as known plasma proteins by several methods including micro amino acid analysis of the proteins extracted from the stained gel pieces. Human cerebrospinal fluid and urine protein patterns were compared with the normalized map and the positions of proteins characteristic of each body fluid sample were determined.