Biochemical characterization of the Micrurus pyrrhocryptus venom
Biochemical characterization of the Micrurus pyrrhocryptus venom
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DOI：
10.1016/j.toxicon.2008.12.015
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发表时间：
2009-03-01
期刊：
影响因子：
2.8
通讯作者：
de Jimenez Bonino, Mirtha Biscoglio
中科院分区：
文献类型：
--
作者：
Christian Dokmetjian, Jose;del Canto, Sergio;de Jimenez Bonino, Mirtha Biscoglio
蛇毒毒性是多肽和蛋白质结合的结果，其鉴定和表征对于理解蛇毒中毒和开发新的临床治疗方法具有重要意义。Elapinae亚科包括珊瑚蛇，其咬伤主要引起神经毒性作用，使肌肉收缩丧失，心脏麻痹，以及抑制呼吸。然而，毒液毒素的结构-功能关系仅针对少数物种进行了研究。我们在此研究Micrurus pyrrhocyptus毒液的生物学方面，如LD 50，出血。坏死、凝血、肌毒和溶血活性以及毒液成分与α-银环蛇毒素竞争尼古丁乙酰胆碱受体配体结合位点的能力。此外，我们还报道了毒素的分子量和N-末端序列的测定，包括PLA 2s，短，长和弱神经毒素。还获得了一种短神经毒素的完整序列，这是在M. pyrrhocyptus毒液和一个少数完全确定的成员Micrurus属。(C)2008爱思唯尔有限公司保留所有权利。
Snake venom toxicity is the consequence of a combination of peptides and proteins whose identification and characterization are of great importance to understand envenomation and develop new clinical treatments. The Elapinae subfamily includes coral snakes whose bite causes mainly neurotoxic effects which disable muscle contraction and paralyse the heart as well as inhibit respiration. However, the structure-function relationship of venom toxins has been investigated only for a few species. We herein study biological aspects of the Micrurus pyrrhocryptus venom such as LD50, hemorrhagic. necrotic, coagulant, myotoxic and hemolytic activity as well as the ability of venom components to compete with alpha-Bungarotoxin for the ligand-binding site of the nicotinic acetylcholine receptor. Besides, we report the determination of the molecular mass and N-terminal sequence of toxins including PLA2s, short, long and weak neurotoxins. The complete sequence of one of the short neurotoxins has also been obtained, this being the first sequence of an alpha-neurotoxin determined in the M. pyrrhocryptus venom and one of the few fully determined in members of the Micrurus genus. (C) 2008 Elsevier Ltd. All rights reserved.