DIFFERENTIAL GENE-REGULATION BY ESTROGEN AND PROGESTERONE IN THE PRIMATE ENDOMETRIUM
DIFFERENTIAL GENE-REGULATION BY ESTROGEN AND PROGESTERONE IN THE PRIMATE ENDOMETRIUM
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DOI：
10.1016/0303-7207(95)03674-v
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发表时间：
1995-11-30
影响因子：
4.1
通讯作者：
OKULICZ, WC
中科院分区：
文献类型：
--
作者：
ACE, CI;OKULICZ, WC
在恒河猴月经周期从增生型子宫内膜向分泌型子宫内膜转变的过程中，黄体酮的作用引起了大量的代谢和结构重塑。为了鉴定在从雌激素(E)优势到孕酮(P)优势的变化中具有潜在重要意义的基因表达，我们使用半定量、逆转录聚合酶链反应（RT-PCR）启动了一项特定基因调控的研究。从e显性（人工月经周期[AMCs]第9-13天）和p显性（第21-23天）恒河猴子宫内膜中分离到PolyA + RNA。将这两个mRNA池转化为cDNA，末端连接到双链寡核苷酸接头上，并使用接头互补引物进行PCR扩增。这一过程产生了E-和PcDNA模板群体，用于dna特异性筛选和PCR比较定量。初步分析表明，胎盘蛋白14 （PP14）依赖于P，人补体3 （HC3）在E显性组织中表达上调，而b -肌动蛋白和甘油醛-3-磷酸脱氢酶（G-3-PDH）在E和P显性组织中表达水平相当。E受体（ER）、P受体（PR）、表皮生长因子受体（EGFR）和胰岛素样生长因子（IGF-I）在E或P优势下的表达相当。p -显性组织中表皮生长因子（EGF）和视网膜母细胞瘤（RE）表达下调。相反，IGF-1受体（IGF-1- r）、转化生长因子- β 2 （TGFB-2）、TGFB-2受体（TGFB-2- r）、17 β -羟基类固醇脱氢酶（17- b - hsd）和白血病抑制因子（LIF）水平在PcDNA中上调。其中，PP14、LIF、IGF-1-R、TGFB-2和17-B-HSD在p依赖性cDNA文库中均可检测到。这些数据为特定基因产物的激素调节提供了证据，这些基因产物可能在灵长类动物子宫内膜的正常成熟和着床准备中发挥重要作用。
During the shift from a proliferative to a secretory endometrium in the rhesus menstrual cycle, progesterone action causes massive metabolic and structural remodelling. In order to identify genes whose expression is potentially important for the change from estrogen (E) to progesterone (P) dominance we have initiated a study of specific gene regulation using semiquantitative, reverse transcription polymerase chain reaction (RT-PCR). PolyA + RNA was isolated from both E-dominant (days 9-13 of artificial menstrual cycles [AMCs]) and P-dominant (days 21-23) rhesus monkey endometria. The two pools of mRNA were converted to cDNA, end-ligated to double-stranded oligonucleotide adaptors and amplified by PCR using an adaptor-complementary primer. This procedure resulted in the production of E- and PcDNA template populations for cDNA-specific screening and comparative quantitation by PCR. Initial analysis showed that placental protein 14 (PP14) was P-dependent and human complement 3 (HC3) was up-regulated in E-dominant tissue, whereas the housekeeping genes B-actin and glyceraldehyde-3-phosphate dehydrogenase (G-3-PDH) were expressed at equivalent levels under E and P dominiance. Expression of the E receptor (ER), P receptor (PR), epidermal growth factor receptor (EGFR) and insulin-like growth factor (IGF-I) was equivalent under E or P dominance. Expression of epidermal growth factor (EGF) and retinoblastoma (RE) was down-regulated in P-dominant tissue. Conversely IGF-1 receptor (IGF-1-R), transforming growth factor-beta 2 (TGFB-2), TGFB-2 receptor (TGFB-2-R), 17 beta-hydroxysteroid dehydrogenase (17-B-HSD) and leukemia inhibitory factor (LIF) levels were up-regulated in PcDNA. Among these factors, PP14, LIF, IGF-1-R TGFB-2 and 17-B-HSD were also detectable by PCR in a P-dependent cDNA library isolated by subtractive hybridization. These data provide evidence for hormonal regulation of specific gene products that may play important roles in the normal maturation of the primate endometrium in preparation for implantation.