Effect of temperature, hypoxia and disinfection with ozonated seawater during somitogenesis on muscular development of the trunk in larval seven-band grouper, Epinephelus septemfasciatus.
Effect of temperature, hypoxia and disinfection with ozonated seawater during somitogenesis on muscular development of the trunk in larval seven-band grouper, Epinephelus septemfasciatus.
复制标题
体节发生过程中温度、缺氧和臭氧海水消毒对七带石斑鱼幼体躯干肌肉发育的影响。
DOI：
10.1111/are.12425
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发表时间：
2014
影响因子：
2
通讯作者：
奥沢公一
中科院分区：
文献类型：
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作者：
宇治督;鈴木徹;岩崎孝史;照屋和久;平沢敬一;白樫真;尾上静正;山下洋一;辻正治;土橋康史;奥沢公一
在人工培养的七带石斑鱼（Epinealphus septemfasciatus）（Thunberg）幼虫中，同一肌节两侧肌肉组织异常的原因尚不清楚。我们评估了三个因素的影响：温度变化（在22和24°C之间），用臭氧化海水消毒（0.3 mg TROS L−1，1分钟），以及在早期（受精后20 h，hpf）和中期（24 hpf）体节阶段的缺氧（22或14%饱和度）对七带石斑鱼幼虫躯干肌肉发育的影响。臭氧化海水消毒和热处理对肌肉发育没有影响（P> 0.05，G-检验）。相比之下，在早期和中期体节阶段暴露于缺氧会增加躯干异常肌肉组织的发生率（P<0.01，G检验）。这些幼虫在躯干两侧有异常的肌肉组织。此外，异常肌节的数量在这些幼虫中不同。在早期和中期暴露分别导致第11和第13节肌节异常的频率最高。我们的研究结果表明，在体节发育过程中的缺氧可以导致异常的肌肉发育在人工培养的七带石斑鱼幼虫的躯干。
The cause of abnormal musculature on both sides of the same myotome in artificially cultured seven‐band grouperEpinephelus septemfasciatus(Thunberg) larvae remains unclear. We evaluated the effect of three factors: changes in temperature (between 22 and 24°C), disinfection with ozonated seawater (0.3 mg TROs L−1, 1 min), and hypoxia (22 or 14% saturation) during the early (20 h post‐fertilization, hpf) and mid‐ (24 hpf) somite stages on trunk muscular development in larval seven‐band grouper. The thermal regimes and disinfection with ozonated seawater had no effect on muscle development (P> 0.05,G‐test). In contrast, exposure to hypoxia during both the early and mid‐somite stages increased the incidence of abnormal musculature in the trunk (P< 0.01,G‐test). These larvae had abnormal musculature on both sides of the trunk. In addition, the number of abnormal myotomes differed among these larvae. Exposure in the early and mid‐somite stages resulted in the highest frequency of abnormalities in the 11th and 13th myotome respectively. Our results suggest that hypoxia during somitogenesis can lead to abnormal muscle development in the trunk of artificially cultured seven‐band grouper larva.