Dipolar Janus liposomes: formation, electrokinetic motion and self-assembly
Dipolar Janus liposomes: formation, electrokinetic motion and self-assembly
复制标题
偶极 Janus 脂质体：形成、动电运动和自组装
DOI：
10.1039/c9sm02254f
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发表时间：
2020
期刊：
影响因子：
3.4
通讯作者：
Zhan, Wei
中科院分区：
文献类型：
--
作者：
Liu, Zening;Cui, Jinyan;Zhan, Wei
本文首次报道了偶极Janus脂质体--含有相反的表面电荷装饰同一胶体的两个半球的脂质体。这种表面电荷的异质组织是通过胆固醇调节的脂相分离实现的，它将阴离子/阳离子脂类分类为共存的液体有序/液体无序结构域。基于胆固醇、1，2-二棕榈酰-sn-甘油-3-磷胆碱和1，2-二油酰-sn-甘油-3-磷胆碱三元脂质体系的凝胶辅助水合作用，我们提出了高产率生产这些脂质体的最佳实验条件。通过共聚焦荧光显微镜、纳米球结合和Zeta电位测量详细地表征了这些脂质体的尺寸/电荷分布和结构域构型。利用共聚焦荧光显微镜，我们还跟踪了这些新的偶极Janus粒子的电动运动以及静电自组装。
Presented herein is the first report on dipolar Janus liposomes–liposomes that contain opposite surface charges decorating the two hemispheres of the same colloidal body. Such heterogeneous organization of surface charge is achieved through cholesterol-modulated lipid phase separation, which sorts anionic/cationic lipids into coexisting liquid-ordered/liquid-disordered domains. We present optimized experimental conditions to produce these liposomes in high yields, based on the gel-assisted hydration of ternary lipid systems consisting of cholesterol, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, and 1,2-dioleoyl-sn-glycero-3-phosphocholine. The size/charge distribution and domain configuration of these liposomes are characterized in detail by confocal fluorescence microscopy, nanosphere binding and zeta potential measurements. Using confocal fluorescence microscopy, we also follow the electrokinetic motion as well as the electrostatic self-assembly of these new dipolar Janus particles.