CHLORHEXIDINE DIACETATE AND POVIDONE-IODINE CYTO-TOXICITY TO CANINE EMBRYONIC FIBROBLASTS AND STAPHYLOCOCCUS-AUREUS
CHLORHEXIDINE DIACETATE AND POVIDONE-IODINE CYTO-TOXICITY TO CANINE EMBRYONIC FIBROBLASTS AND STAPHYLOCOCCUS-AUREUS
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DOI：
10.1111/j.1532-950x.1988.tb00995.x
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发表时间：
1988-07-01
影响因子：
1.8
通讯作者：
MCGUIRE, JA
中科院分区：
文献类型：
--
作者：
SANCHEZ, IR;NUSBAUM, KE;MCGUIRE, JA
评价了醋酸氯己定和聚维酮碘对犬胚胎成纤维细胞原代细胞系的成纤维细胞毒性，以及对金黄色葡萄球菌的杀菌效力。培养的成纤维细胞或S.将金黄色葡萄球菌暴露于0.5至0.0005%二乙酸氯己定、5.0至0.05%聚维酮碘或作为对照的生理缓冲盐水的递增稀释液中30分钟。为了确定存活率，将成纤维细胞胰蛋白酶化并计数;在脑心浸液琼脂上计数金黄色葡萄球菌菌落。两组的存活率通过计算测试稀释液中活细胞数量与对照培养物中活细胞数量的百分比来表示。成纤维细胞存活发生在氯己定浓度小于0.013%和聚维酮碘浓度小于0.5%时（p < 0.05）。重要的S。当氯己定浓度小于0.05%和聚维酮碘浓度小于1.0%时，金黄色葡萄球菌存活率显著高于对照组（P < 0.05）。这些数据表明，所有杀菌浓度的双醋酸氯己定和聚维酮碘在体外对犬胚胎成纤维细胞是致死的，而非致死浓度允许显著的细菌存活。
Chlorhexidine diacetate and povidone-iodine were evaluated for fibroblast toxicity on a primary line of canine embryonic fibroblasts, and for bactericidal efficacy against Staphylococcus aureus. The cultured fibroblasts or S. aureus were exposed for 30 minutes to incremental dilutions of 0.5 to 0.0005% chlorhexidine diacetate, 5.0 to 0.05% povidone-iodine, or physiologic buffered saline as a control. To determine survival, fibroblasts were trypsinized and counted; S. aureus colonies were counted on brain-heart infusion agar. Survival for both groups was expressed by calculating the number of living cells in test dilutions as a percentage of the number in control cultures. Fibroblast survival occurred at chlorhexidine concentrations less than 0.013% and at povidone-iodine concentrations less than 0.5% (p < 0.05). Significant S. aureus suvival (P < 0.05) was noted at chlorhexidine concentrations less than 0.05% and povidone-iodine concentrations less than 1.0%. These data showed that all bactericidal concentrations of chlorhexidine diacetate and povidone-iodine were lethal to canine embryonic fibroblasts in vitro, whereas non-lethal concentrations allowed significant bacterial suvival.