Expression of fatty acid binding protein 4 is involved in the cell growth of oral squamous cell carcinoma
Expression of fatty acid binding protein 4 is involved in the cell growth of oral squamous cell carcinoma
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DOI：
10.3892/or.2014.2975
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发表时间：
2014-03-01
期刊：
影响因子：
4.2
通讯作者：
Kogo, Mikihiko
中科院分区：
文献类型：
--
作者：
Lee, Doksa;Wada, Koichiro;Kogo, Mikihiko
脂肪酸结合蛋白(FABP)是一类高度保守的小分子脂质伴侣分子，具有多种功能。其中，脂肪细胞、巨噬细胞和树突状细胞高表达脂肪酸结合蛋白4(FABP4，又称aP2)。虽然FABP4在癌症中的作用尚不清楚，但据报道，它在卵巢癌和膀胱癌等人类肿瘤中高度表达。本研究探讨FABP4在口腔鳞癌及其组织中的表达和作用。免疫组织化学染色显示，FABP4在同一标本的肿瘤组织中的表达明显高于非肿瘤区域。在体外研究中，FABP4基因敲除的SCC细胞系(通过FABP4特异性siRNA建立)显示生长受到抑制，丝裂原活化蛋白激酶(MAPK)的表达和激活受到抑制。这些结果表明，FABP4的表达通过MAPK通路在口腔鳞状细胞癌的细胞生长中起重要作用。
Fatty acid binding proteins (FABPs) are a family of small and highly conserved lipid chaperone molecules with highly varied functions. Among them, fatty acid binding protein 4 (FABP4, also known as aP2) is highly expressed by adipocytes, macrophages and dendritic cells. Although the role of FABP4 in cancer is still unclear, it has been reported to be highly expressed by human tumors such as ovarian and bladder cancers. In the present study, we investigated the expression and role of FABP4 in oral squamous cell carcinoma (SCC) and its expression in oral SCC tissues. Immunohistochemical staining revealed that FABP4 expression in the tumor tissue was much higher than that in the non-tumor area of the same specimen. In the in vitro studies, an FABP4-knockdown SCC cell line (established through FABP4-specific siRNA) showed inhibited growth, and inhibited expression and activation of mitogen-activated protein kinase (MAPK). These results indicate that expression of FABP4 plays an important role in the cell growth of oral SCC through the MAPK pathway.