MOLECULAR-CLONING OF A MEMBER OF A NEW CLASS OF LOW-MOLECULAR-WEIGHT GTP-BINDING PROTEINS
MOLECULAR-CLONING OF A MEMBER OF A NEW CLASS OF LOW-MOLECULAR-WEIGHT GTP-BINDING PROTEINS
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DOI：
10.1101/gad.5.12b.2386
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发表时间：
1991-12-01
影响因子：
10.5
通讯作者：
KOSHLAND, DE
中科院分区：
文献类型：
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作者：
MORIMOTO, BH;CHUANG, CC;KOSHLAND, DE
我们报告的低分子量的GTP结合蛋白，似乎是一个新的G蛋白类的第一个成员的克隆。这种G蛋白是从HT 4神经细胞系克隆的，与低分子量（LMW）G蛋白家族的rab、sec 4和ypt 1成员具有最接近的同源性。氨基酸序列的同一性是只有30%，与这些其他的低分子量G蛋白，但在四个保守的GTP结合结构域，氨基酸的同一性增加到50- 100%。将该G蛋白与其他LMW G蛋白区分开的独特特征是其羧基末端氨基酸序列-Cys-Cys-Pro。为了与ras超家族的其他成员保持一致，我们将这个新的类别命名为rah（ras相关同源物）。基于序列同源性，rah可能在囊泡运输和神经递质分泌中起作用。
We report the cloning of a low-molecular-weight GTP-binding protein that appears to be the first member of a new class of G proteins. This G protein was cloned from the HT4 neural cell line and has the closest homology to the rab, sec4, and ypt1 members of the low-molecular-weight (LMW) G-protein family. The amino acid sequence identity is only 30% with these other LMW G proteins, but in the four conserved GTP-binding domains, amino acid identity increases to 50-100%. A unique feature that distinguishes this G protein from other LMW G proteins is its carboxy-terminal amino acid sequence -Cys-Cys-Pro. In keeping with the current nomenclature for other members of the ras superfamily, we will designate this new class as rah (ras-related homolog). On the basis of sequence homology, rah may function in vesicular trafficking and possibly in neurotransmitter secretion.