Characterization and expression pattern of r-spondin1 in Cynoglossus semilaevis
Characterization and expression pattern of r-spondin1 in Cynoglossus semilaevis
复制标题
半滑舌鱼 r-spondin1 的特征和表达模式。
DOI：
10.1002/jez.b.22774
复制
发表时间：
2017-12-01
影响因子：
2.2
通讯作者：
Li, Yangzhen
中科院分区：
文献类型：
--
作者：
Li, Hailong;Xu, Wenteng;Li, Yangzhen
r-spondin 1（rspo 1）编码一种分泌蛋白，其参与哺乳动物卵巢的决定和分化。然而，关于硬骨鱼类的信息还很少。在这里，我们确定了同源rspo 1在半滑舌鳎。rspo 1基因的cDNA全长为2，703 bp，开放阅读框为834 bp，编码277个氨基酸。通过qRT-PCR检测到rspo 1在各种组织中的表达，并且在性腺中观察到显著的性二态性表达。此外，ISH定位rspo 1在生殖细胞，如精原细胞，精母细胞，精子细胞，精子和卵母细胞，以及在性腺的体细胞。在卵巢细胞系中敲低rspo 1后，wnt 4a、β-catenin、foxl 2和星星的表达受到高度影响; wnt 4a和β-catenin显著下调，而foxl 2和星星显著上调。总之，这些数据表明，rspo 1可能参与卵巢发育和分化的调节，通过一个保守的途径，而在睾丸中的基因的功能仍然是难以捉摸的。
r-spondin1 (rspo1) encodes a secreted protein that is involved in the determination and differentiation of the mammalian ovary. However, little information is yet available for teleosts. Here, we identified a homologue of rspo1 in Cynoglossus semilaevis. The full-length cDNA of rspo1 had a length of 2,703bp with an open reading frame of 834bp, encoding a protein with a length of 277 amino-acids. rspo1 expression was detected via qRT-PCR in various tissues, and significant sexually dimorphic expression was observed in the gonads. Furthermore, ISH located rspo1 in germ cells such as spermatogonia, spermatocytes, spermatids, spermatozoa, and oocytes, as well as in somatic cells of the gonads. Following knockdown of rspo1 in an ovarian cell line, the expressions of wnt4a, -catenin, foxl2, and StAR were highly affected; wnt4a and -catenin were significantly downregulated, whereas foxl2 and StAR were significantly upregulated. In summary, these data suggest that rspo1 may be involved in the regulation of ovarian development and differentiation through a conserved pathway, while the function of the gene in the testis remains elusive.