Ribosome Fingerprinting with a Solid-State Nanopore
Ribosome Fingerprinting with a Solid-State Nanopore
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DOI：
10.1101/2020.08.07.236406
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发表时间：
2020-08
期刊：
影响因子：
8.9
通讯作者：
Mukhil Raveendran;Anna R. Leach;T. Hopes;J. Aspden;P. Actis
中科院分区：
文献类型：
--
作者：
Mukhil Raveendran;Anna R. Leach;T. Hopes;J. Aspden;P. Actis
纳米孔具有在单一实体水平上分析复杂生物分子的巨大潜力。一个特别有趣的大分子机器是核糖体，负责将信使核糖核酸翻译成蛋白质。在这项研究中，我们使用固态纳米孔对人类神经元细胞系以及果蝇培养的细胞和卵巢中的80S核糖体和多聚体进行指纹图谱分析。具体地说，我们发现80S核糖体的峰值幅度和停留时间特征与多聚体不同，可以用来区分混合样品中的核糖体和多聚体。此外，我们能够区分含有7个以上核糖体的大多聚体和含有2-3个核糖体的多聚体，并证明了多聚体大小与峰值幅度之间的相关性。这项研究强调了固态纳米孔作为检测和表征核糖体复合体的快速分析工具的应用。
Nanopores hold great potential for the analysis of complex biological molecules at the single entity level. One particularly interesting macromolecular machine is the ribosome, responsible for translating mRNA into proteins. In this study, we use a solid-state nanopore to fingerprint 80S ribosomes and polysomes from a human neuronal cell line and, Drosophila melanogaster cultured cells and ovaries. Specifically, we show that the peak amplitude and dwell time characteristics of 80S ribosomes are distinct from polysomes and can be used to discriminate ribosomes from polysomes in mixed samples. Moreover, we are able to distinguish large polysomes, containing more than 7 ribosomes, from those containing 2-3 ribosomes, and demonstrate a correlation between polysome size and peak amplitude. This study highlights the application of solid-state nanopores as a rapid analytical tool for the detection and characterization of ribosomal complexes.