Statistical exploration of variation in quantitative two‐dimensional gel electrophoresis data
Statistical exploration of variation in quantitative two‐dimensional gel electrophoresis data
复制标题
定量二维凝胶电泳数据变化的统计探索
作者：
John S. Gustafsson;Robert Ceasar;C. Glasbey;A. Blomberg;M. Rudemo
双向凝胶电泳法是蛋白质水平全球分析的一种主要技术。本文介绍了用放射性标记的酿酒酵母蛋白对三种凝胶组的斑点体积数据的检查。通过应用移位对数变换，数据中的强方差对均值相关性被发现是稳定的。然而，转换后的数据显示，不同蛋白质仍然存在显著的差异异质性。此外，对学生化残差的检验表明，转换后的数据近似为正态分布，并且凝胶中的测量误差之间存在空间相关性。
Two‐dimensional gel electrophoresis is a major technique in global analysis at the protein level. This paper presents an examination of spot volume data from three gel sets with radioactively labeled yeast Saccharomyces cerevisiae proteins. A strong variance versus mean dependence in data was found to be stabilized by applying a shifted logarithmic transformation. However, transformed data showed a remaining substantial variance heterogeneity for different proteins. Furthermore, examination of studentized residuals revealed that transformed data were approximately normally distributed and that there were spatial correlations among the measurement errors in the gel.
用于二维凝胶定量分析的 QUEST 系统。
DOI：
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发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Garrels,JI
通讯作者：
Garrels,JI