Plasma levels of vascular endothelial growth factor are increased in patients with metastatic prostate cancer
Plasma levels of vascular endothelial growth factor are increased in patients with metastatic prostate cancer
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DOI：
10.1016/s0090-4295(99)00167-3
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发表时间：
1999-09-01
期刊：
影响因子：
2.1
通讯作者：
Freeman, MR
中科院分区：
文献类型：
--
作者：
Duque, JLF;Loughlin, KR;Freeman, MR
目标。血管内皮生长因子(VEGF)是一种在肿瘤血管生成中发挥重要作用的细胞因子，在包括前列腺癌在内的许多人类肿瘤中均有过表达，但在前列腺癌患者循环中的水平尚未见报道。在这项研究中，我们分析了一组前列腺癌患者的血浆血管内皮生长因子浓度，并将他们与正常人群进行比较。对26例健康无癌个体和80例前列腺癌患者(54例局限性前列腺癌和26例转移性前列腺癌[骨或淋巴结阳性])进行了分析。以同样的方式从所有个体中提取血液，并将其储存在含有乙二胺四乙酸作为抗凝剂的试管中。提取血浆，用双抗体夹心酶免疫测定法测定血浆中血管内皮生长因子的浓度。转移性疾病患者血浆中位数为28.5pg/mL(四分位数范围19.3~57.0)，局限性疾病患者中位数为7.0pg/mL(四分位数范围0~26.5)，对照组为0 pg/mL(四分位数范围0~24)。经统计学处理，差异有统计学意义(P
Objectives. Vascular endothelial growth factor (VEGF) is a cytokine that plays an important role in tumor angiogenesis, VEGF is overexpressed in many human cancers, including prostate cancer, but circulating levels of VEGF in patients with prostate cancer have not been reported. In this study, we analyzed plasma concentrations of VEGF in a cohort of patients with prostate cancer and compared them with a normal population.Methods. Twenty-six healthy, cancer-free individuals and 80 patients with prostate cancer (54 patients with localized prostate cancer and 26 patients with metastatic prostate cancer [bone or lymph node positive]) were analyzed in this study. Blood was drawn in the same fashion from all individuals and deposited in tubes containing ethylenediaminetetraacetic acid as anticoagulant. Plasma was extracted and VEGF concentrations were determined using a quantitative sandwich enzyme immunoassay technique.Results. Median plasma VEGF was 28.5 pg/mL (interquartile range 19.3 to 57.0) in patients with metastases; 7.0 pg/mL (interquartile range 0 to 26.5) in patients with localized disease, and 0 pg/mL (interquartile range 0 to 24) in controls. These differences were statistically significant (P