Development of the Zebrafish Mesonephros
Development of the Zebrafish Mesonephros
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DOI：
10.1002/dvg.22846
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发表时间：
2015-03-01
期刊：
影响因子：
1.5
通讯作者：
Davidson, Alan J.
中科院分区：
文献类型：
--
作者：
Diep, Cuong Q.;Peng, Zhenzhen;Davidson, Alan J.
脊椎动物的肾脏在清除代谢废物和平衡水盐方面起着至关重要的作用。这是由肾元来完成的，肾元包括附着在上皮小管上的血液过滤器，具有近端和远端部分。在斑马鱼中，首先形成两个肾单位作为胚胎肾脏的一部分（原肾单位），然后形成数百个肾单位组成成年肾脏（中肾单位）。以前的研究主要集中在前肾的发育上，而对中肾是如何形成的知之甚少。在这里，我们描述了斑马鱼中肾细胞的发育，并检查了在幼虫变态过程中形成的肾细胞。这些肾单位产生于增殖的祖细胞，表达肾转录因子基因wt1b、pax2a和lhx1a，形成于肾原小管顶部，并形成与肾原肾单位相似的分割模式。我们发现原肾充当中肾的支架，在中肾中，新的肾细胞与肾原小管的远端部分融合，形成最终的分支网络，这是成年斑马鱼肾脏的特征。创世记53:257-269,2015。(c) 2015 Wiley期刊公司
The vertebrate kidney plays an essential role in removing metabolic waste and balancing water and salt. This is carried out by nephrons, which comprise a blood filter attached to an epithelial tubule with proximal and distal segments. In zebrafish, two nephrons are first formed as part of the embryonic kidney (pronephros) and hundreds are formed later to make up the adult kidney (mesonephros). Previous studies have focused on the development of the pronephros while considerably less is known about how the mesonephros is formed. Here, we characterize mesonephros development in zebrafish and examine the nephrons that form during larval metamorphosis. These nephrons, arising from proliferating progenitor cells that express the renal transcription factor genes wt1b, pax2a, and lhx1a, form on top of the pronephric tubules and develop a segmentation pattern similar to pronephric nephrons. We find that the pronephros acts as a scaffold for the mesonephros, where new nephrons fuse with the distal segments of the pronephric tubules to form the final branching network that characterizes the adult zebrafish kidney. genesis 53:257-269, 2015. (c) 2015 Wiley Periodicals, Inc.