Identification of a G protein-coupled receptor for pheromone biosynthesis activating neuropeptide from pheromone glands of the moth Helicoverpa zea
Identification of a G protein-coupled receptor for pheromone biosynthesis activating neuropeptide from pheromone glands of the moth Helicoverpa zea
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DOI：
10.1073/pnas.1632485100
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发表时间：
2003-08-19
影响因子：
11.1
通讯作者：
Jurenka, R
中科院分区：
文献类型：
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作者：
Choi, MY;Fuerst, EJ;Jurenka, R
信息素生物合成激活神经肽（PBAN）是由食道下神经节产生的一种肽，被多种蛾类用来调节信息素的产生。PBAN通过钙离子和cAMP作为第二信使直接作用于信息素腺细胞。我们已经确定了一个基因编码的G蛋白偶联受体（GPCR）的雌蛾信息素腺体。该基因基于与来自果蝇的一组GPCR的序列同一性来鉴定，所述GPCR与脊椎动物中的神经介肽U受体同源。随后克隆全长PBAN受体，在Sf 9昆虫细胞中表达，并显示响应于PBAN动员钙。该响应具有剂量依赖性（EC 50 = 25 nM），在300 nM时响应最大，在10 nM时可观察到最小响应。还测试了由PBAN编码基因产生的另外四种肽的活性，并且确定三种具有与PBAN相似的活性，而另一种活性略低。属于与PBAN相同家族的肽，即焦激肽，以及脊椎动物神经介肽U肽也诱导钙应答。我们已经确定了一个GPCR的PBAN/pyrokinin家族的肽具有已知的功能，刺激信息素生物合成的雌蛾。它与来自昆虫（果蝇和按蚊）的几种受体以及来自脊椎动物的神经介肽U和饥饿素受体相关。
Pheromone biosynthesis-activating neuropeptide (PBAN), a peptide produced by the subesophageal ganglion, is used by a variety of moths to regulate pheromone production. PBAN acts directly on pheromone gland cells by using calcium and cAMP as second messengers. We have identified a gene encoding a G protein-coupled receptor (GPCR) from pheromone glands of the female moth Helicoverpa zea. The gene was identified based on sequence identity to a group of GPCRs from Drosophila that are homologous to neuromedin U receptors in vertebrates. The full-length PBAN receptor was subsequently cloned, expressed in Sf9 insect cells, and shown to mobilize calcium in response to PBAN. This response was dose-dependent (EC50 = 25 nM) with a maximum response at 300 nM and a minimal observable response at 10 nM. Four additional peptides produced by the PBAN-encoding gene were also tested for activity, and it was determined that three had similar activity to PBAN and the other was slightly less active. Peptides belonging to the same family as PBAN, namely pyrokinins, as well as the vertebrate neuromedin U peptide also induced a calcium response. We have identified a GPCR for the PBAN/pyrokinin family of peptides with a known function of stimulating pheromone biosynthesis in female moths. It is related to several receptors from insects (Drosophila and Anopheles) and to neuromedin U and ghrelin receptors from vertebrates.