Evaluation of red blood cells stored at-80°C in excess of 10 years
Evaluation of red blood cells stored at-80°C in excess of 10 years
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DOI：
10.1111/j.1537-2995.2004.03271.x
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发表时间：
2004-09-01
期刊：
影响因子：
2.9
通讯作者：
Acker, JP
中科院分区：
文献类型：
--
作者：
Lecak, J;Scott, K;Acker, JP
背景：在40% （wt/vol）甘油中冷冻的红细胞目前被FDA和AABB批准在-80℃下储存长达10年。研究设计和方法：本研究检测了20个红细胞单位，这些单位在40% （wt/vol）甘油中冷冻保存，并在-80度下保存长达22年。冻融洗（FTW）回收率、ATP、2,3- dpg、高铁血红蛋白、红细胞指数、形态学和渗透脆性在脱甘油后和在4℃下储存24小时后立即测定。结果：冷冻10年以上的红细胞平均FTW恢复正常，氧转运功能正常，红细胞形态、红细胞指数、高铁血红蛋白和渗透脆弱性正常。统计分析表明，冷冻保存红细胞的体外活力和功能与冷冻保存或解冻后4℃的保存时间无关，而与冷冻前4℃的保存时间有关。结论：本研究报告的数据表明，红细胞可以在-80℃下保存10年以上，体外质量可接受，并建议采用更明确的冷冻保存标准。
BACKGROUND: RBCs frozen in 40 percent (wt/vol) glycerol are currently approved by the FDA and the AABB for storage at -80degreesC for up to 10 years.STUDY DESIGN AND METHODS: This study examined 20 RBC units that had been cryopreserved in 40 percent (wt/vol) glycerol and stored at -80degreesC for up to 22 years. Measures of the freeze-thaw-wash (FTW) recovery, ATP, 2,3-DPG, methemoglobin, RBC indices, morphology, and osmotic fragility were made immediately after deglycerolization and after 24 hours of storage at 4degreesC.RESULTS: RBCs frozen for longer than 10 years had acceptable mean FTW recovery, normal oxygen transport function, RBC morphology, RBC indices, methemoglobin, and osmotic fragility. Statistical analysis indicated that the in-vitro viability and function of cryopreserved RBCs was not dependent on the length of frozen storage or postthaw storage at 4degreesC but did correlate with the storage length at 4degreesC before cryopreservation.CONCLUSION: The data reported in this study demonstrate that RBCs can be stored at -80degreesC beyond 10 years with acceptable in-vitro quality and suggest that more defined criteria for the cryopreservation process be adopted.