Purification and characterization of Eikenella corrodens aggregating factor from submandibular-sublingual saliva.
Purification and characterization of Eikenella corrodens aggregating factor from submandibular-sublingual saliva.
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下颌下舌下唾液中腐蚀艾肯菌聚集因子的纯化和表征。
DOI：
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发表时间：
1988
影响因子：
3.5
通讯作者：
H. Okada
中科院分区：
文献类型：
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作者：
S. Ebisu;H. Fukuhara;H. Okada
艾肯氏菌腐蚀1073在实验动物中已被证明会诱发牙周病，当与人类下颌-舌下（SM-SL）唾液混合时，发现它会聚集。本研究的目的是分离、纯化和化学表征E.腐蚀菌细胞的凝集物质（E.腐蚀菌聚集因子[Ec-AF]）。所有供者的SM-SL唾液中均检测到Ec-AF，而腮腺唾液中未检测到E.腐蚀聚集活性。采用ecteqla -纤维素柱层析、超滤和凝胶过滤高效液相色谱法从SM-SL唾液中纯化Ec-AF。纯化后的Ec-AF比活性提高了81倍，产率为16.7%。在十二烷基硫酸钠和β-巯基乙醇中对Ec-AF进行聚丙烯酰胺凝胶电泳，得到一个亚基分子量为140000的蛋白条带。用周期性酸-希夫试剂处理相同的凝胶，结果只有与考马斯蓝染色带一致的物质染色，表明Ec-AF是一种糖蛋白。化学分析表明，Ec-AF由蛋白质（27.3%）、中性糖（30.3%）、己糖（24.9%）和唾液酸（17.3%）组成。Ec-AF多肽骨架含有33%的丝氨酸加苏氨酸和36%的脯氨酸加丙氨酸。Ec-AF的碳水化合物部分由n -乙酰半乳糖、半乳糖、焦糖、唾液酸、n -乙酰氨基葡萄糖组成，其摩尔比约为2:2:1:1:1。
Eikenella corrodens 1073, which has been shown to induce periodontal disease in experimental animals, was found to aggregate when mixed with human submandibular-sublingual (SM-SL) saliva. The purpose of this study was to isolate, purify and chemically characterize the substance that agglutinates E. corrodens cells (E. corrodens aggregating factor [Ec-AF]). Ec-AF was detected in SM-SL saliva of all the donors tested, whereas E. corrodens aggregating activity was not detected in parotid saliva. Ec-AF was purified from SM-SL saliva by ECTEQLA-cellulose column chromatography, ultrafiltration and gel filtration high performance liquid chromatography. An 81-fold increase in the specific activity of purified Ec-AF was achieved with a yield of 16.7%. Polyacrylamide gel electrophoresis of Ec-AF in sodium dodecy) sulfate and β-mercaptoethanol produced a single protein band corresponding to a subunit molecular weight of 140 000. Treatment of an identical gel with periodic acid-Schiff reagent resulted in staining only of material coinciding with the Coomassie blue-staining band, indicating that the Ec-AF was a glycoprotein. Chemical analysis indicated that Ec-AF is composed of protein (27.3%), neutral sugars (30.3%), hexosamines (24.9%), and sialic acid (17.3%). The polypeptide backbones of Ec-AF contained 33% serine plus threonine and 36% proline plus alanine. The carbohydrate moety of Ec-AF consisted of N-acetylgalactosamine, galactose, fucose, sialic acid, N-acetylglucosamine, with their molar ratio being approximately 2:2:1:1:1, respectively.
某些龈下微生物物种在选定牙周条件下的分布。
影响因子：
3.5
作者：
Savitt,ED;Socransky,SS
通讯作者：
Socransky,SS
从人颌下舌下唾液中纯化低分子量粘蛋白型糖蛋白。
影响因子：
3.1
作者：
Prakobphol,A;Levine,MJ;Tabak,LA;Reddy,MS
通讯作者：
Reddy,MS