Probing RNA-Protein Interactions and RNA Compaction by Sedimentation Velocity Analytical Ultracentrifugation.
Probing RNA-Protein Interactions and RNA Compaction by Sedimentation Velocity Analytical Ultracentrifugation.
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通过沉降速度分析超速离心探测 RNA-蛋白质相互作用和 RNA 压实。
DOI：
10.1007/978-1-0716-0278-2_19
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Demeler,Borries
中科院分区：
文献类型：
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作者：
Mitra,Somdeb;Demeler,Borries
多波长分析超速离心法(MWL-AUC)的最新进展结合了基于流体力学的精密灵敏分离技术的力量和增加了光谱分离的维度。这一增加的维度为更好地描述溶液中多种相互作用的物种打开了新的大门。当应用于RNA的结构研究时，MWL-AUC可以精确地报告RNA分子的流体动力学半径和整体形状，从而能够精确地测量其沉降和扩散系数，并基于光谱分解识别相互作用组分的化学计量比。本章提供的信息将使研究人员能够设计实验，以探测离子和/或蛋白质引起的RNA分子的全局构象变化，并利用蛋白质和RNA之间的光谱差异来表征它们在生理溶液环境中的相互作用。
Recent advances in multi-wavelength analytical ultracentrifugation (MWL-AUC) combine the power of an exquisitely sensitive hydrodynamic-based separation technique with the added dimension of spectral separation. This added dimension has opened up new doors to much improved characterization of multiple, interacting species in solution. When applied to structural investigations of RNA, MWL-AUC can precisely report on the hydrodynamic radius and the overall shape of an RNA molecule by enabling precise measurements of its sedimentation and diffusion coefficients and identify the stoichiometry of interacting components based on spectral decomposition. Information provided in this chapter will allow an investigator to design experiments for probing ion and/or protein-induced global conformational changes of an RNA molecule and exploit spectral differences between proteins and RNA to characterize their interactions in a physiological solution environment.