THE RATE OF CALCIUM EXTRACTION DURING EDTA DECALCIFICATION FROM THIN BONE SLICES AS ASSESSED WITH ATOMIC-ABSORPTION SPECTROPHOTOMETRY
THE RATE OF CALCIUM EXTRACTION DURING EDTA DECALCIFICATION FROM THIN BONE SLICES AS ASSESSED WITH ATOMIC-ABSORPTION SPECTROPHOTOMETRY
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DOI：
10.1007/bf00489507
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
HELMINEN, HJ
中科院分区：
文献类型：
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作者：
KIVIRANTA, I;TAMMI, M;HELMINEN, HJ
用原子吸收光谱仪测定了EDTA从300µm-2 mm厚的兔骨片中提取钙的速率。用15%EDTA(0.40 M)、8%(0.22 M)和4%(0.11 M)EDTA分别在24 h、3 d和5 d(体积15ml，温度4度)将0.5 mm厚的骨片完全脱钙。C，pH 7.4)。在37度和60度。C脱矿速度较20℃略有加快。C级，而4级和20级之间无差异。C.将0.3、0.5、1和2 mm的骨片按相同顺序脱钙，时间分别为24小时、2天、3天和5天(8%EDTA，4度)。C，pH 7.4)。PH为7.4时，脱钙速度略慢于pH为5.0和8.5时。除体积很小时外，搅拌不影响脱钙速度，也不影响药剂的体积。普通骨，包括致密骨和松质骨，其脱矿速度比先前报道的同质骨更快。作为参照物的酸性缓冲液和新十钙比EDTA具有更强的脱钙效果。甲酸钾/甲酸缓冲液，pH 3.15，1 mm厚的骨片脱矿时间为24 h；乳酸钠/乳酸缓冲液，pH为3.70，需要2天。在pH为0.9的新十钙溶液中，1.5h即可完成相应的脱矿。原子吸收光谱仪是评价骨片中钙提取速度的一种有用工具。
The rate of Ca extraction with EDTA from thin [rabbit] bone slices (300 .mu.m-2 mm thick) was determined by an atomic absorption spectrophotometer. A 0.5 mm thick bone slice was completely decalcified with 15% (0.40 M), 8% (0.22 M), and 4% (0.11 M) EDTA in 24 h, 3 days and 5 days, respectively, (volume 15 ml, temperature 4.degree. C, pH 7.4). At 37 and 60.degree. C the speed of demineralization was slightly increased as compared with that at 20.degree. C, while no difference was observed between 4 and 20.degree. C. Bone slices with a thickness of 0.3, 0.5, 1 and 2 mm were decalcified, in the same order, in 24 h, 2, 3 and 5 days (8% EDTA, 4.degree. C, pH 7.4). At pH 7.4, the decalcification rate was a little slower than at pH 5.0 and 8.5. Agitation did not affect the decalcifying velocity, nor did the volume of the agent, except when the volume was very small. The demineralization of ordinary bone, containing both compact and spongy bone, was more rapid than that of homogeneous bone reported earlier. The acidic buffers and New Decalc, which served as reference substances, exerted a more vigorous decalcifying effect than EDTA. K formate/formic acid buffer, pH 3.15, demineralized a 1 mm thick bone slice in 24 h, and 2 days was needed with Na lactate/lactic acid buffer, pH 3.70. With New Decalc, pH 0.9, the corresponding demineralization was accomplished in 1.5 h. Atomic absorption spectrophotometer is a useful tool in the evaluation of Ca extraction velocity from bone slices.