AMD3100 Accelerates Reendothelialization of Neointima in Rabbit Saccular Aneurysm After Flow Diverter Treatment.
AMD3100 Accelerates Reendothelialization of Neointima in Rabbit Saccular Aneurysm After Flow Diverter Treatment.
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AMD3100 加速分流器治疗后兔囊状动脉瘤的新内皮化。
DOI：
10.1016/j.wneu.2017.07.128
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发表时间：
2017-11
影响因子：
2
通讯作者：
Liu Jianmin
中科院分区：
文献类型：
--
作者：
Li Zifu;Zhao Rui;Fang Xinggen;Zhou Jiahao;Jiang Guoquan;Huang Qinghai;Liu Jianmin
目的观察基质细胞衍生因子1/CXC趋化因子受体4（stromal cell-derived factor-1/CXC chemokine receptor 4，SCF-1/CXC receptor 4）拮抗剂AMD 3100对血流导向装置（flow diverter，FD）治疗兔囊状动脉瘤后新生内膜形成的影响。3周后，将Tubridge FD植入囊状动脉瘤中。所有处理的模型立即分为2组：AMD 3100组皮下注射AMD 3100（5 mg/kg/d），对照组注射生理盐水。FD治疗后2周和4周，使用硬组织切片和Masson三色染色研究新生内膜的形态和厚度。扫描电镜观察内皮样细胞的形态，荧光定量聚合酶链反应（PCR）检测新生内膜生物标志物激酶插入结构域受体、VE-钙粘蛋白、CD 34、Tie 2的mRNA表达。结果FD治疗后2周、4周，AMD 3100组新生内膜内皮样细胞数量明显多于对照组。Masson三色染色显示AMD 3100组新生内膜较对照组完整、增厚。此外，增加的激酶插入结构域受体，VE-钙粘蛋白，Tie 2在新生内膜的mRNA水平被发现在AMD 3100组与control group.ConclusionsInterval使用AMD 3100促进兔囊状动脉瘤新生内膜的形成，并促进FD治疗后的新生内膜内皮化。
ObjectiveWe sought to inspect the role of AMD3100, which acts as an antagonist of stromal cell–derived factor-1/CXC chemokine receptor 4 on the formation of neointima in rabbit saccular aneurysm after flow diverter (FD) treatment.MethodsTwenty saccular aneurysm models were established by using porcine pancreatic elastase. Three weeks later, a Tubridge FD was implanted into the saccular aneurysm. All treated models were immediately divided into 2 groups: the AMD3100 group was subcutaneously injected with AMD3100 (5 mg/kg per day), while the control group received saline. Morphology and thickness of the neointima were investigated 2 and 4 weeks after FD treatment, using hard tissue section and masson trichrome staining. Scanning electron microscope was used to observe endothelial-like cells, and fluorescence quantitative polymerase chain reaction was used to determine mRNA expression of neointima biomarkers, such as kinase insert domain receptor, VE-cadherin, CD34, and Tie2.ResultsTwo and 4 weeks after FD treatment, the AMD3100 group had more endothelial-like cells than the control group in the neointima. Masson trichrome staining showed that the neointima in the AMD3100 group was more intact and thicker than that in the control group. Furthermore, increased mRNA levels of kinase insert domain receptor, VE-cadherin, and Tie2 in the neointima were found in the AMD3100 group compared with the control group.ConclusionsInterval use of AMD3100 promotes the formation of neointima in rabbit saccular aneurysm and facilitates the endothelialization of the neointima after FD treatment.
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