Integrated circuit/microfluidic chip to programmably trap and move cells and droplets with dielectrophoresis
Integrated circuit/microfluidic chip to programmably trap and move cells and droplets with dielectrophoresis
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DOI：
10.1039/b710928h
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发表时间：
2008-01-01
期刊：
影响因子：
6.1
通讯作者：
Westervelt, R. M.
中科院分区：
文献类型：
--
作者：
Hunt, Thomas P.;Issadore, David;Westervelt, R. M.
我们提出了一个集成电路/微流控芯片，捕获和移动个别活的生物细胞和化学液滴沿着可编程的路径，使用介电泳（DEP）。我们的芯片将微流体的生物相容性与集成电路（IC）的可编程性和复杂性相结合。该芯片能够同时独立控制数千个介电物体的位置，如细胞和化学液滴。该芯片由一个128 × 256像素的阵列组成，尺寸为11 × 11 μ m（2），由内置SRAM存储器控制;每个像素可以由高达5 Vpp的射频（RF）电压供电。集成电路是在一家商业铸造厂制造的，微流体室是在哈佛的顶面制造的。使用这种混合芯片，我们已经移动酵母和哺乳动物细胞通过微流控室的速度高达30 μ m秒（-1）。成千上万的细胞可以单独捕获，并同时定位在受控模式。该芯片可以捕获并移动油中的pL水滴，将一滴分成两滴，并将两滴混合成一滴。我们的IC/微流控芯片提供了一个多功能平台，可以单独捕获和移动大量细胞和液滴，用于芯片实验室应用。
We present an integrated circuit/microfluidic chip that traps and moves individual living biological cells and chemical droplets along programmable paths using dielectrophoresis (DEP). Our chip combines the biocompatibility of microfluidics with the programmability and complexity of integrated circuits (ICs). The chip is capable of simultaneously and independently controlling the location of thousands of dielectric objects, such as cells and chemical droplets. The chip consists of an array of 128 x 256 pixels, 11 x 11 mu m(2) in size, controlled by built-in SRAM memory; each pixel can be energized by a radio frequency (RF) voltage of up to 5 Vpp. The IC was built in a commercial foundry and the microfluidic chamber was fabricated on its top surface at Harvard. Using this hybrid chip, we have moved yeast and mammalian cells through a microfluidic chamber at speeds up to 30 mu m sec(-1). Thousands of cells can be individually trapped and simultaneously positioned in controlled patterns. The chip can trap and move pL droplets of water in oil, split one droplet into two, and mix two droplets into one. Our IC/microfluidic chip provides a versatile platform to trap and move large numbers of cells and fluid droplets individually for lab-on-a-chip applications.