Cell proliferation in the Rana catesbeiana auditory medulla over metamorphic development.
Cell proliferation in the Rana catesbeiana auditory medulla over metamorphic development.
复制标题
林蛙听觉延髓中的细胞增殖超过变态发育。
DOI：
10.1002/neu.20209
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Simmons,AndreaMegela
中科院分区：
文献类型：
--
作者：
Chapman,JudithA;Weinstein,JoshuaL;Simmons,AndreaMegela
在变态发育过程中，牛蛙(Rana Catesbeiana)在形态、解剖和生理方面都发生了实质性的变化，因为它们正在为从完全水生到半陆生的转变做准备。利用BrdU掺入和免疫组织化学方法，我们定量研究了幼虫发育过程中两个关键的听性脑干核团--背外侧核和上橄榄核--细胞增殖的变化。从孵化到早期幼虫阶段，两个细胞核中的增殖细胞数量增加，与可用于标记的细胞总数的总体增加相平行。上橄榄核内的增殖细胞数量在幼虫晚期和聋期减少，在变态高潮时显著增加。背外侧核内的增殖细胞数量从孵化到仔鱼后期逐渐增多，聋期逐渐减少，高潮期逐渐增多。在这两个核中，尽管可用于标记的细胞数量增加，但在变性后的Froglet阶段，增殖细胞的数量减少。新生成的细胞分别在注射BrdU后1周和1个月开始表达神经胶质或神经特异性表型，而一些新细胞在有丝分裂后2天内表达γ-氨基丁酸。我们的数据显示，在建立基于大气听觉的转导系统之前，这些听觉核团显著上调有丝分裂。©2005 Wiley期刊，Inc.神经生物学杂志，2005
During metamorphic development, bullfrogs(Rana catesbeiana)undergo substantial morphological, anatomical, and physiological changes as the animals prepare for the transition from a fully‐aquatic to a semi‐terrestrial existence. Using BrdU incorporation and immunohistochemistry, we quantify changes in cell proliferation in two key auditory brainstem nuclei, the dorsolateral nucleus and the superior olivary nucleus, over the course of larval and early postmetamorphic development. From hatchling through early larval stages, numbers of proliferating cells increase in both nuclei, paralleling the overall increase in total numbers of cells available for labeling. Numbers of proliferating cells in the superior olivary nucleus decrease during the late larval and deaf periods, and significantly increase during metamorphic climax. Proliferating cells in the dorsolateral nucleus increase in number from hatchling to late larval stages, decrease during the deaf period, and increase during climax. In both nuclei, numbers of proliferating cells decrease during the postmetamorphic froglet stage, despite increases in the number of cells available for label. Newly generated cells express either glial‐ or neural‐specific phenotypes beginning between 1 week and 1 month post‐BrdU injection, respectively, while some new cells express γ‐aminobutyric acid within 2 days of mitosis. Our data show that these auditory nuclei dramatically up‐regulate mitosis immediately prior to establishment of a transduction system based on atmospheric hearing. © 2005 Wiley Periodicals, Inc. J Neurobiol, 2005