Physical-chemical-morphological characterization of the whole sugarcane lignocellulosic biomass used for 2G ethanol production by spectroscopy and microscopy techniques
Physical-chemical-morphological characterization of the whole sugarcane lignocellulosic biomass used for 2G ethanol production by spectroscopy and microscopy techniques
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DOI：
10.1016/j.renene.2015.10.054
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发表时间：
2016-03-01
期刊：
影响因子：
8.7
通讯作者：
Farinas, Cristiane S.
中科院分区：
文献类型：
--
作者：
Pereira, Sandra C.;Maehara, Larissa;Farinas, Cristiane S.
甘蔗木质纤维素生物质的自然顽抗性仍然是第二代（2G）乙醇生产的挑战。本文评估了商业甘蔗品种的整个甘蔗木质纤维素生物质（包括甘蔗渣、秸秆和顶部）在稀硫酸预处理前后的物理化学形态特征，以帮助预测这些材料在 2G 乙醇过程中的行为。使用 NMR、FTIR、XRD 和 SEM 进行的分析表明，此处评估的甘蔗品种的特性非常相似。对于不同的残留部分，根据 XRD 结果计算出的结晶度指数值也相似，并且由于半纤维素的去除，在预处理后更高。甘蔗渣的木质素和结晶纤维素 FTIR 吸收带最强烈，其次是稻草和顶部。 NMR 分析确定了骨架芳香族和甲氧基的存在，归因于木质素结构，信号强度遵循以下顺序：甘蔗渣 > 秸秆 > 顶部。 SEM 图像显示结构破坏的顺序为：顶部 > 秸秆 > 甘蔗渣。光谱和形态差异有助于阐明使甘蔗渣中的甘蔗渣部分不易受酶糖化影响的特征。秸秆和顶部光谱之间的差异表明，秸秆不易被酶促消化，形态分析也表明了这一点。结果表明，光谱学和显微镜技术的结合使用有助于了解用于 2G 乙醇生产的不同生物质的行为。 (C) 2015 Elsevier Ltd. 保留所有权利。
The natural recalcitrance of sugarcane lignocellulosic biomass remains a challenge for second generation (2G) ethanol production. Here, the physical chemical morphological characteristics of the whole sugarcane lignocellulosic biomass (including bagasse, straw, and tops) from commercial sugarcane varieties were evaluated before and after dilute sulfuric acid pretreatment, in order to help predict the behaviors of these materials during the 2G ethanol process. Analyses using NMR, FTIR, XRD, and SEM showed that the properties of the sugarcane varieties evaluated here were very similar. The crystallinity index values calculated from the XRD results were also similar for the different residue fractions, and were higher after pretreatment due to the removal of hemicellulose. The lignin and crystalline cellulose FTIR absorption bands were most intense for bagasse, followed by straw and tops. NMR analysis identified the presence of skeletal aromatic and methoxyl groups, attributed to the lignin structure, with the intensity of the signals following the order: bagasse > straw > tops. SEM images showed that structural disruption followed the order: tops > straw > bagasse. The spectral and morphological differences helped to elucidate the characteristics that made the bagasse fraction of the sugarcane residue less susceptible to enzymatic saccharification. Differences between the spectra for straw and tops indicated that the straw was less easily digested by enzymatic action, as also indicated by the morphological analysis. The results demonstrate that the combined use of spectroscopy and microscopy techniques can contribute to understanding the behavior of different biomasses intended to be used for 2G ethanol production. (C) 2015 Elsevier Ltd. All rights reserved.