Purification, characterization and antibacterial potential of a lectin isolated from Apuleia leiocarpa seeds
Purification, characterization and antibacterial potential of a lectin isolated from Apuleia leiocarpa seeds
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DOI：
10.1016/j.ijbiomac.2015.02.001
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发表时间：
2015-04-01
影响因子：
8.2
通讯作者：
dos Santos Correia, Maria Tereza
中科院分区：
文献类型：
--
作者：
Carvalho, Aline de Souza;da Silva, Marcia Vanusa;dos Santos Correia, Maria Tereza
罗汉果树是在卡廷加发现的一种树，在木材工业中具有很大的价值。凝集素是一种碳水化合物结合蛋白，具有多种生物技术应用。本研究用几丁质柱从150 mM氯化钠的粗提物中分离得到多果沙枣种子凝集素(ApulSL)，并对其进行了分离、鉴定和抗菌活性研究。ApulSL吸附在基质上，用1.0M冰醋酸洗脱。天然ApulSL是一个55.8 kDa的酸性蛋白。SOS-PAGE显示三条多肽条带，而双向电泳法显示四个斑点。MALDI TOF/TOF检测到的多肽没有显示出足够的同源性(
Apuleia leiocarpa is a tree found in Caatinga that has great value in the timber industry. Lectins are carbohydrate-binding proteins with several biotechnological applications. This study shows the isolation, characterization, and antibacterial activity of A. leiocarpa seed lectin (ApulSL), The lectin was chromatographically isolated from a crude extract (in 150 mM NaCl) by using a chitin column. ApulSL adsorbed to the matrix and was eluted using 1.0 M acetic acid. Native ApulSL was characterized as a 55.8-kDa acidic protein. SOS-PAGE showed three polypeptide bands, whereas two-dimensional electrophoresis revealed four spots. The peptides detected by MALDI TOF/TOF did not show sufficient homology (