Preferred CDRH3 lengths for antibodies with defined specificities
Preferred CDRH3 lengths for antibodies with defined specificities
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DOI：
10.1093/intimm/10.12.1801
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发表时间：
1998-12-01
影响因子：
4.4
通讯作者：
Wu, TT
中科院分区：
文献类型：
--
作者：
Johnson, G;Wu, TT
重链可变区的第三互补决定区（CDRH3）赋予抗体精细的特异性。所有人或所有小鼠抗体的CDRH3长度分布似乎或多或少是随机的，其中人分布具有更宽的分布。由于Kabat数据库的比对的氨基酸和核苷酸序列的蛋白质的免疫学的兴趣已经增长了至少4倍，在过去的7年中，我们试图研究CDRH3长度分布的人和小鼠抗体与定义的特异性，其中足够的完整和不同的CDRH3序列是可用的。对于针对小的半抗原或碳水化合物的小鼠抗体，对于给定的特异性，优选的长度通常占主导地位。没有足够的人类小半抗原或碳水化合物抗体序列进行类似的研究。人和小鼠抗蛋白抗体都具有更宽范围的CDRH 3长度分布，这可能是由于蛋白分子上存在不同的抗原表位，因此存在许多不同的抗体。对于抗DNA抗体，人和小鼠都表现出甚至更宽的长度分布，因为DNA可以是双链、单链、超螺旋等。
The third complementarity determining regions of heavy chain Variable regions (CDRH3) confer the fine specificities of antibodies. The CDRH3 length distribution of all human or all mouse antibodies appears to be more or less random, with the human distribution having a wider spread. Since the Kabat database of aligned amino and nucleotide sequences of proteins of immunological interest has grown at least 4 times during the past 7 years, we attempt to study CDRH3 length distributions of human and mouse antibodies with defined specificities, where enough complete and distinct CDRH3 sequences are available. For mouse antibodies against small haptens or carbohydrates, a preferred length usually dominates for a given specificity. There are not enough human sequences of small hapten or carbohydrate antibodies for a similar study. Both human and mouse anti-protein antibodies have wider ranges of CDRH3 length distributions, probably due to the presence of different antigenic epitopes on the protein molecules, and thus many different antibodies. For anti-DNA antibodies, both humans and mice exhibit even wider length distributions, since DNA can be double stranded, single stranded, supercoiled, etc.