Biotechnological conversions of bio-diesel derived waste glycerol into added-value compounds by higher fungi: production of biomass, single cell oil and oxalic acid
Biotechnological conversions of bio-diesel derived waste glycerol into added-value compounds by higher fungi: production of biomass, single cell oil and oxalic acid
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DOI：
10.1016/j.indcrop.2009.12.011
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发表时间：
2010-03-01
影响因子：
5.9
通讯作者：
Papanikolaou, Seraphim
中科院分区：
文献类型：
--
作者：
Andre, Axel;Diamantopoulou, Panagiota;Papanikolaou, Seraphim
高等真菌以废弃生物柴油甘油为唯一碳源，两株香菇菌株在碳限碳条件下摇瓶培养，在弱搅拌、pH 4.0、温度25℃的条件下生长良好，最大生物量为5.2g/L。合成的菌丝体每克生物量含有约0.1g脂肪，亚油酸((Delta 9，12)C8：2)是产生的主要细胞脂肪酸。两株黑曲霉菌株在氮气限制的摇瓶培养中培养，其中氮素恒定，两种不同的初始甘油浓度加入培养基中。在250毫升的摇瓶培养中，与在2-L培养瓶中进行的试验形成对比，开发出了大尺寸的颗粒。氮素限制导致草酸分泌和细胞内脂肪积累；无论如何，都可以观察到脂肪和草酸的顺序产生。最初，氮素限制导致了脂肪的积累。此后，累积的脂肪被再次消耗，大量的草酸被分泌到培养液中。在大颗粒中，细胞内总脂含量较高，草酸含量较低，反之亦然。最高草酸含量为20.5-21.5g/L，脂肪含量为3.1-3.5g/L(相当于每克生物质含脂肪0.41-0.57g)。脂肪主要由油酸((Delta 9)C18：1)和亚油酸((Delta 9)C18：2)组成。(C)2010爱思唯尔B.V.保留所有权利。
Waste bio-diesel derived glycerol was used as the sole carbon source by higher fungi; two Lentinula edodes strains were flask cultured in carbon-limited conditions and displayed satisfactory growth in media presenting weak agitation, pH 4.0 and temperature 25 degrees C. Maximum biomass of 5.2g/l was produced. Mycelia were synthesized, containing around 0.1 g of fat per g of biomass, with linoleic acid ((Delta 9,12)C8:2) being the principal cellular fatty acid produced. Two Aspergillus niger strains were grown in nitrogen-limited flask cultures with constant nitrogen and two different initial glycerol concentrations into the medium. In 250-ml flask cultures, large-sized pellets were developed, in contrast with the trials performed in 2-l flasks. Nitrogen limitation led to oxalic acid secretion and intra-cellular lipid accumulation; in any case, sequential production of lipid and oxalic acid was observed. Initially, nitrogen limitation led to lipid accumulation. Thereafter, accumulated lipid was re-consumed and oxalic acid, in significant quantities, was secreted into the medium. In large-sized pellets, higher quantities of intra-cellular total lipid and lower quantities of oxalic acid were produced and vice versa. Maximum quantities of oxalic acid up to 20.5-21.5 g/l and lipid up to 3.1-3.5 g/l (corresponding to 0.41-0.57 g of fat per g of biomass) were produced. Lipid was mainly composed of oleic ((Delta 9)C18:1) and linoleic ( (Delta 9,12)C18:2) acids. (C) 2010 Elsevier B.V. All rights reserved.