Microdialysis evidence that acetylcholine in the nucleus accumhens is involved in morphine withdrawal and its treatment with clonidine
Microdialysis evidence that acetylcholine in the nucleus accumhens is involved in morphine withdrawal and its treatment with clonidine
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DOI：
10.1016/0006-8993(91)91616-9
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发表时间：
1991-10
期刊：
影响因子：
2.9
通讯作者：
P. Rada;E. Pothos;G. P. Mark;B. Hoebel
中科院分区：
文献类型：
--
作者：
P. Rada;E. Pothos;G. P. Mark;B. Hoebel
本研究采用微透析法测定了自由活动大鼠在急性和慢性吗啡处理过程中以及纳洛酮催促戒断后，延髓核（NAC）细胞外乙酰胆碱（ACh）含量的变化。吗啡注射液（20 mg/kg，i.p.）引起细胞外ACh的显著降低，这在重复暴露于阿片制剂7天后不明显。基础恢复乙酰胆碱没有改变慢性吗啡治疗。在吗啡依赖建立后的第8天，纳洛酮引起ACh水平大幅增加，并伴有戒断症状，如湿狗抖动、腹泻和牙齿打颤。可乐定（200 μ g/kg，i.p.）减少了这些戒断症状并消除了ACh反应。这些结果表明，乙酰胆碱参与阿片类药物戒断的一些令人厌恶的方面。
This study used microdialysis to measure changes in extracellular acetylcholine (ACh) content in the nucleus accumbens (NAC) of freely moving rats during acute and chronic morphine treatment, and following naloxone-precipitated withdrawal. Morphine injection (20 mg/kg, i.p.) caused a significant decrease in extracellular ACh which was not apparent after repeated exposure to the opiate for 7 days. Basal recovery of ACh was not altered by chronic morphine treatment. On day 8, after morphine dependence had been established, naloxone caused a large increase in ACh levels accompanied by withdrawal symptoms such as wet dog shakes, diarrhea and teeth-chattering. Pretreatment with clonidine (200 μg/kg, i.p.) reduced these withdrawal symptoms and eliminated the ACh response. These results suggest that accumbens ACh is involved in some of the aversive aspects of opiate withdrawal.