A new mycorrhizal helper bacterium, Ralstonia species, in the ectomycorrhizal symbiosis between Pinus thunbergii and Suillus granulatus
A new mycorrhizal helper bacterium, Ralstonia species, in the ectomycorrhizal symbiosis between Pinus thunbergii and Suillus granulatus
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DOI：
10.1007/s00374-008-0340-0
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发表时间：
2009-02-01
影响因子：
6.5
通讯作者：
Futai, Kazuyoshi
中科院分区：
文献类型：
--
作者：
Kataoka, Ryota;Futai, Kazuyoshi
在日本鸟取县以刺槐为主的沿海森林中，黑松幼苗的生长和存活以及黑松的天然更新受到了R.刺槐。为了促进鸟取沙丘上紫菀幼苗的生长，本研究试图从鸟取沙丘紫菀的菌根环境中寻找一种菌根辅助细菌。从分离自雷公藤根际的9种细菌中筛选出2种MHB，分别为Ralstonia sp.和Bacillus subtilis。采用对峙试验和微宇宙试验研究了细菌对外生菌根真菌颗粒乳牛肝菌的影响。对峙试验表明，Ralstonia sp.促进了S.颗粒状的此外，S. Ralstonia sp.和B对颗粒藻-雷氏藻共生有显著的促进作用。枯草芽孢杆菌Ralstonia sp.和B.枯草芽孢杆菌（P.subtilis）为与雷帕霉素相关的MHB。这是首次报道Ralstonia sp.作为MHB。
In a Robinia-pseudoacacia-dominated coastal forest in Tottori prefecture Japan, the growth and survival of Pinus thunbergii seedlings and the natural regeneration of P. thunbergii was disturbed by R. pseudoacacia. In order to improve the growth of P. thunbergii seedling in the Tottori sand dune, we tried to find a mycorrhiza helper bacteria (MHB) from P. thunbergii mycorrhizosphere in a Tottori sand dune. Two MHB, Ralstonia sp. and Bacillus subtilis, were selected from the nine bacterial species isolated from the mycorrhizosphere of P. thunbergii. The bacterial effect on the ectomycorrhizal fungus Suillus granulatus was investigated by confrontation assay and a microcosm experiment. The confrontation assay showed that Ralstonia sp. promoted the hyphal growth of S. granulatus. Moreover, the S. granulatus-P. thunbergii symbiosis was significantly stimulated by Ralstonia sp. and B. subtilis. Ralstonia sp. and B. subtilis were regarded as MHB associated with P. thunbergii. This is the first report of Ralstonia sp. as an MHB.